Therapeutic Efficacy of Allyl Isothiocyanate Evaluated on N-Nitrosodiethylamine/Phenobarbital induced Hepatocarcinogenesis in Wistar Rats


  • A. S. Gowtham Kumar Genetics Division, Central Research Laboratory, Chettinad University, Kelambakkam, Chennai-603103, India.
  • G. Thiyagarajan Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai-600 020, India.
  • V. Ramakrishnan Department of Pharmacology, Chettinad University, Kelambakkam, Chennai-603103, India.
  • N. Madhusudhanan Department of Pharmacology, Sri Manakula Vinayagar Medical College and Hospital, Pondicherry-605107, India.
  • C. Anbu Selvam Department of Pharmacology and Environmental Toxicology, Dr. A.L.M.P.G.I.B.M.S, University of Madras, Taramani, Chennai-600113, India.


Hepatocarcinogenesis, Allyl isothiocyanate, Antioxidants, Marker enzymes, Alfa-fetoprotein


N-nitrosodiethylamine (NDEA) is a potential carcinogenic agent that induces liver cancer. To evaluate the chemotherapeutic effect of Allyl isothiocyanate in the experimental model, Wistar male rats were administered single dose of intraperitoneal (IP) injection of NDEA. Two weeks after administration of NDEA, Phenobarbital at the concentration of 0.05% was incorporated in rat chow for up to 14 successive weeks to promote liver cancer. Allyl isothiocyanate (AITC) (2mg/kg body weight) in addition with 0.5ml of corn oil was given orally on a daily basis. At the end of this experimental period, the rats were sacrificed and the blood samples were taken for biochemical studies. The levels of the marker enzymes for liver function were measured in serum. The results of the biochemical studies showed that NDEA administration followed by phenobarbital induces macro and microscopic liver tumors that increase the levels of marker enzymes and decreases the level of antioxidant in the serum in addition to loss of body weight. Conclusively, the administration of AITC as therapeutic treatment for hepatocarcinoma has significantly reduced the tumor development and counteracted all the biochemical effects induced by NDEA.


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How to Cite

Gowtham Kumar, A. S., Thiyagarajan, G., Ramakrishnan, V., Madhusudhanan, N., & Selvam, C. A. (2010). Therapeutic Efficacy of Allyl Isothiocyanate Evaluated on N-Nitrosodiethylamine/Phenobarbital induced Hepatocarcinogenesis in Wistar Rats. Advances in BioScience, 1(1), 5–9. Retrieved from