Sero-Surveillance of Mycobacterium avium subspecies paratuberculosis Infection in Domestic Livestock in North India Using Indigenous Absorbed ELISA Test

Authors

  • A. V. Singh Microbiology Laboratory, Animal Health Division, CIRG, Makhdoom, PO Farah, Mathura- (UP), India.
  • S. V. Singh Microbiology Laboratory, Animal Health Division, CIRG, Makhdoom, PO Farah, Mathura- (UP), India.
  • P. K. Singh Microbiology Laboratory, Animal Health Division, CIRG, Makhdoom, PO Farah, Mathura- (UP), India.
  • J. S. Sohal Microbiology Laboratory, Animal Health Division, CIRG, Makhdoom, PO Farah, Mathura- (UP), India.
  • K. Mahour Microbiology Laboratory, Animal Health Division, CIRG, Makhdoom, PO Farah, Mathura- (UP), India.

Keywords:

Johne's disease, Paratuberculosis, Mycobacterium avium subspecies paratuberculosis, ELISA, Seroprevalence

Abstract

A total of 829 serum samples belonging to domestic livestock (Cattle, buffaloes, goat and sheep) and driven from different parts of North India between 2005 to 2008, were screened to estimate the seroprevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infection using 'indigenous absorbed ELISA kit'. Seroprevalence of MAP in the domestic livestock was 23.1%. Prevalence was higher in large ruminants (24.1%) as compared to small ruminants (22.5%). Highest seropositivity was in cattle (26.9%), followed by goats (23.9%), buffaloes (20.2%), and sheep (19.0%). In cattle region-wise, 25.8, 29.1 and 30.7% animals were positive from Mathura (UP), Rohtak (Haryana), and Bareilly (UP) regions, respectively. In buffaloes, the highest prevalence was found at Bareilly (26.6%) followed by Rohtak (20.0%) and Bhaghpat (18.4%) regions. In goats, 19.6, 37.5, 40.0 and 21.9% animals were positive from Mathura (farm herd), Etawah, Agra and Ajmer (farmers herd) regions, respectively. In sheep, prevalence of MAP was 25.5 and 16.3% in Mathura and Mannavanur regions, respectively. In sheep, prevalence was higher in Northern region as compared to the Southern region of the country. The present study showed that the prevalence of MAP in domestic livestock was moderately higher; therefore there is an urgent need to control the disease at National level in order to improve per animal productivity in the country.

References

Barbaruah, M.I. & Joseph, A.K. (2008). India: Dairy giant walking barefoot. Proceedings of the FAO-RAP Technical meeting on Designing Effective Country-Specific Dairy Strategies, November 17-20, 2008, FAO, Rome, Italy.

Chiodini, R.J., Van Kruiningen, H.J. & Merkal, R.S. (1984). Ruminant paratuberculosis (Johne's disease): the current status and future prospects. Cornell Vet., 74(3): 218–262.

Collins M.T. (2002). Interpretation of a commercial bovine paratuberculosis enzyme-linked immunosorbent assay by using likelihood ratios. Clin. Diagn. Lab. Immunol., 9(6): 1367–1371. https://doi.org/10.1128/cdli.9.6.1367-1371.2002.

Hasonova, L. & Pavlik, I. (2006). Economic impact of paratuberculosis in dairy cattle herds: a review . Vet. Med., 51: 193-211. https://doi.org/10.17221/5539-VETMED.

Kumar, P., Singh, S.V., Bhatiya, A.K., Sevilla, I., Singh, A.V., Whittington, R.J., Juste, R.A., Gupta, V.K., Singh, P.K., Sohal, J.S. & Vihan, V.S. (2007). Juvenile Capri-Paratuberculosis (JCP) in India: Incidence and characterization by six diagnostic tests. Small Rumin. Res., 73: 45–53. http://dx.doi.org/10.1016/j.smallrumres.2006.10.023.

Rideout, B.A., Brown, S.T., Davis, W.C., Gay, J.M., Giannella, R.A., Hines, M.E., Heuston, W.D. & Hutchinson, L.J. (2003). Diagnosis and Control of Johne's Disease. The National Academies Press, Washington, D.C.

Sharma, G., Singh, S.V., Sevilla, I., Singh, A.V., Whittington, R.J., Juste, R.A., Kumar, S., Gupta, V.K., Singh, P.K., Sohal, J.S. & Vihan, V.S. (2008). Evaluation of indigenous milk ELISA with m-culture and m-PCR for the diagnosis of bovine Johne's disease (BJD) in lactating Indian dairy cattle. Res. Vet. Sci., 84(1): 30–37. https://doi.org/10.1016/j.rvsc.2007.03.014.

Singh, A.V., Singh, S.V., Sohal, J.S. & Singh, P.K. (2009). Comparative potential of modified indigenous, indigenous and commercial ELISA kits for diagnosis of Mycobacterium avium subspecies paratuberculosis in goat and sheep. Indian J. Exp. Biol., 47(5): 379–382.

Singh, N., Singh, S.V., Gupta, V.K., Sharma, V.D., Sharma, R.K. & Katoch, V.M. (1996). Isolation and identification of Mycobacterium paratuberculosis from naturally infected goatherds in India. Indian J. Vet. Pathol., 20: 104-108.

Singh, S.V., Singh, A.V., Singh, P.K. & Sohal, J.S. (2008b). An outbreak of caprine Paratuberculosis in a newly established commercial goat farm. Haryana Vet., 47: 110-112.

Singh, S.V., Singh, A.V., Singh, P.K., Sohal, J.S. & Singh, N.P. (2007). Evaluation of an indigenous ELISA for diagnosis of Johne's disease and its comparison with commercial kits. Indian J. Microbiol., 47(3): 251–258. https://doi.org/10.1007/s12088-007-0046-2.

Singh, S.V., Singh, A.V., Singh, R., Sharma, S., Shukla, N., Misra, S., Singh, P.K., Sohal, J.S., Kumar, H., Patil, P.K., Misra, P. & Sandhu, K.S. (2008a). Sero-prevalence of bovine Johne's disease in buffaloes and cattle population of North India using indigenous ELISA kit based on native Mycobacterium avium subspecies paratuberculosis 'Bison type' genotype of goat origin. Comp. Immunol. Microbiol. Infect. Dis., 31(5): 419–433. https://doi.org/10.1016/j.cimid.2007.06.002.

Sivakumar, P., Tripathi, B.N. & Singh, N. (2005). Detection of Mycobacterium avium subsp. paratuberculosis in intestinal and lymph node tissues of water buffaloes (Bubalus bubalis) by PCR and bacterial culture. Vet. Microbiol., 108: 263–270. https://doi.org/10.1016/j.vetmic.2005.04.002.

Vohra, J., Singh, S.V., Singh, A.V., Singh, P.K. & Sohal, J.S. (2008). Comparative distribution of Mycobacterium avium subspecies paratuberculosis in the target and non-target tissues of goats and sheep population in India. Indian J. Anim. Sci., 78(4): 342–347.

Whitlock, R.H. & Buergelt, C. (1996). Preclinical and clinical manifestations of paratuberculosis (including pathology). Vet. Clin. North Am. Food Anim. Pract., 12(2): 345–356. https://doi.org/10.1016/s0749-0720(15)30410-2.

Yadav, D., Singh, S.V., Singh, A.V., Sevilla, I., Juste, R.A., Singh, P.K. & Sohal, J.S. (2008). Pathogenic 'Bison-type' Mycobacterium avium subspecies paratuberculosis genotype characterized from riverine buffalo (Bubalus bubalis) in North India. Comp. Immunol. Microbiol. Infect. Dis., 31(4): 373–387. https://doi.org/10.1016/j.cimid.2007.06.007.

Yokomizo, Y., Merkal, R.S. & Lyle, P.A. (1983). Enzyme-linked immunosorbent assay for detection of bovine immunoglobulin G1 antibody to a protoplasmic antigen of Mycobacterium paratuberculosis. Am. J. Vet. Res., 44(11): 2205–2207.

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Published

2010-07-01

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Singh, A. V., Singh, S. V., Singh, P. K., Sohal, J. S., & Mahour, K. (2010). Sero-Surveillance of Mycobacterium avium subspecies paratuberculosis Infection in Domestic Livestock in North India Using Indigenous Absorbed ELISA Test. Advances in BioScience, 1(1), 1–4. Retrieved from http://e-journal.sospublication.co.in/index.php/ab/article/view/15

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Vol. 1 No. 1 (2010): July

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