Evaluation of acute toxicity of Ducetia japonica on mice model
Keywords:Ducetia japonica, Acute toxicity, Entomophagy, Mice
Ducetia japonica, also known as bush cricket, is an edible insect that is valued as a food source by various tribal communities in Arunachal Pradesh. Its nutrient content makes it a novel source for both human food and animal feed. However, no research has been done on its food safety or possible toxicity. The toxicological evaluation was carried out on mice for 2-weeks at four distinct dose levels of 2000, 3000, 4000, and 5000 mg/kg bodyweight, according to the criteria of the Organisation for Economic Co-operation and Development (OECD). Bodyweight and clinical indicators showed no substantial toxicological related alterations. Furthermore, no toxicological changes in haematology were observed. When compared to a vehicle control group, serum alanine aminotransferase and creatinine levels did not differ between the experimental groups. In the liver and kidney tissues of mice, no histological and gross abnormalities were found. The relative organ weight of the treatment groups did not differ significantly from that of the vehicle control group. As a result, the LD50 value for Ducetia japonica is considered to be greater than 5000 mg/kg body weight and no evidence of toxic changes were observed in the present study.
Grafton, R.Q., Daugbjerg, C. & Qureshi, M.E. (2015). Towards food security by 2050. Food Sec., 7(2): 179–183. https://doi.org/10.1007/s12571-015-0445-x.
Patel, S., Suleria, H.A.R. & Rauf, A. (2019). Edible insects as innovative foods: Nutritional and functional assessments. Trends Food Sci. Technol., 86: 352–359. https://doi.org/10.1016/j.tifs.2019.02.033.
Paoletti, M.G. & Dufour, D.L. (2002). Minilivestock. In: Pimental, D. (ed.), Encyclopedia of Pest Management. Marcel Dekker, New York, pp. 487–492.
Paoletti, M.G. (2005). Ecological Implications of Minilivestock: Potential of Insects, Rodents, Frogs and Sails. Science Publishers Inc., Enfield, New Hampshire, pp. 648. https://doi.org/10.1201/9781482294439.
Srivastava, S.K., Babu, N. & Pandey, H. (2009). Traditional insect bioprospecting - As human food and medicine. Indian J. Tradit. Knowl., 8(4): 485–494. http://hdl.handle.net/123456789/6263.
Thakur, A., Thakur, K.S. & Thakur, N.S. (2017). Entomophagy (insects as human food): a step towards food security. National conference on advances in food science and technology: current trends and future perspectives. Himachal Pradesh, India. http://dx.doi.org/10.13140/RG.2.2.29644.72327.
Bodenheimer, F.S. (1951). Insects as Human Food; a Chapter of the Ecology of Man. Dr. W. Junk, Publishers, The Hague, pp. 352. https://doi.org/10.1007/978-94-017-6159-8.
Sponheimer, M., de Ruiter, D., Lee-Thorp, J. & Späth, A. (2005). Sr/Ca and early hominin diets revisited: new data from modern and fossil tooth enamel. J. Hum. Evol., 48(2): 147–156. https://doi.org/10.1016/j.jhevol.2004.09.003.
Mariod, A.A. (2013). Insect oil and protein: Biochemistry, food and other uses: Review. Agric. Sci., 4(9): 76–80. http://dx.doi.org/10.4236/as.2013.49B013.
Rumpold, B.A. & Schlüter, O.K. (2013). Nutritional composition and safety aspects of edible insects. Mol. Nutr. Food Res., 57(5): 802–823. https://doi.org/10.1002/mnfr.201200735.
Di Mattia, C., Battista, N., Sacchetti, G. & Serafini, M. (2019). Antioxidant Activities in vitro of Water and Liposoluble Extracts Obtained by Different Species of Edible Insects and Invertebrates. Front. Nutr., 6: 106. https://doi.org/10.3389/fnut.2019.00106.
Chatsuwan, N., Nalinanon, S., Puechkamut, Y., Lamsal, B.P. & Pinsirodom, P. (2018). Characteristics, Functional Properties, and Antioxidant Activities of Water-Soluble Proteins Extracted from Grasshoppers, Patanga succincta and Chondracris roseapbrunner. J. Chem., 2018: 6528312. https://doi.org/10.1155/2018/6528312.
Dutta, P., Dey, T., Manna, P. & Kalita, J. (2016). Antioxidant Potential of Vespa affinis L., a Traditional Edible Insect Species of North East India. PLoS One, 11(5): e0156107. https://doi.org/10.1371/journal.pone.0156107.
Zielińska, E., Baraniak, B. & Karaś, M. (2017). Antioxidant and Anti-Inflammatory Activities of Hydrolysates and Peptide Fractions Obtained by Enzymatic Hydrolysis of Selected Heat-Treated Edible Insects. Nutrients, 9(9): 970. https://doi.org/10.3390/nu9090970.
Ma, G., Wu, L., Shao, F., Zhang, C. & Wan, H. (2019). Antimicrobial Activity of 11 Insects Extracts Against Multi Drug Resistant (MDR) Strains of Bacteria and Fungus. IOP Conf. Ser.: Earth Environ. Sci., 252(2): 022132. https://doi.org/10.1088/1755-1315/252/2/022132.
Gaikwad, S.M., Koli, Y.J., Raut, G.A., Waghmare, S.H. & Bhawane, G.P. (2016). Long-horned grasshoppers (Orthoptera: Tettigoniidae) in Radhanagari Wildlife Sanctuary, Maharashtra, India. J. Threat. Taxa, 8(2): 8533–8537. https://doi.org/10.11609/jott.25184.108.40.20633-8537.
Chakravorty, J., Gogoi, M., Jugli, S. & Boria, M. (2018). Ducetia japonica and Phyllozelus Sp.: Two Tettigoniid Species of Orthopteran Insects Appreciated by Tribal People of Arunachal Pradesh (North-East India) May Serve as Future Alternative Food Source. Food Nutr. J., 3: 180. https://doi.org/10.29011/2575-7091.100080.
OECD (2001). Test No. 423: Acute Oral toxicity - Acute Toxic Class Method. OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris, pp. 14. https://doi.org/10.1787/9789264071001-en.
Imathiu, S. (2020). Benefits and food safety concerns associated with consumption of edible insects. NFS J., 18: 1–11. https://doi.org/10.1016/j.nfs.2019.11.002.
Durst, P.B., Johnson, D.V., Leslie, R.N. & Shono, K. (eds.) (2010). Forest insects as food: humans bite back. Proceedings of a workshop on Asia-Pacific resources and their potential for development, FAO Regional Office for Asia and the Pacific, Bangkok, Thailand, pp. 73. Available at: http://www.fao.org/3/i1380e/i1380e.pdf.
Van Huis, A. (2016). Edible insects are the future? Conference on ‘The future of animal products in the human diet: health and environmental concerns’. Proceedings of the Nutrition Society, 75(3): 294-305. https://doi.org/10.1017/S0029665116000069.
How to Cite
Copyright (c) 2021 Copyright (c) 2021 The author(s) retains the copyright of this article.
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.