Molecular Characterization of Nepali Potato Cultivars Using Randomly Amplified Polymorphic DNA (Rapd) Markers

  • Dhurva P. Gauchan Department of Biotechnology, Kathmandu University, Dhulikhel-45200, Kavre, Nepal.
  • Chris Brinegar Division of Natural Sciences, University of Maine, Farmington, Maine-04938, USA.
  • Arjan Hada Department of Biotechnology, Kathmandu University, Dhulikhel-45200, Kavre, Nepal.
  • Dipali Singh Department of Biotechnology, Kathmandu University, Dhulikhel-45200, Kavre, Nepal.
Keywords: RAPD, Genetic Diversity, Potato, Solanum tuberosum, Genetic Similarity

Abstract

Randomly amplified polymorphic DNA (RAPD) was used to study the genetic diversity of four local cultivars of potato. Amplification with ten arbitrary decamer primers produced 29 different marker bands of which 69.0% were polymorphic. The size range of the amplified DNAs ranged between 370 bp and 2500 bp. On average, 17.5 alleles per genotype were amplified using the RAPD primers. With the selected primers sufficient polymorphism could be detected to allow identification of individual genotypes. A dendrogram displaying the relative genetic similarities between the genotypes showed a range of 55.2-69.0% similarity.

Downloads

Download data is not yet available.

References

[1]. del Rio, A.H., Bamberg, J.B., Huaman, Z., Salas, A. and Vega, S.E. (2001). Association of ecogeographical variables and RAPD marker variation in wild potato populations of the USA. Crop Sci. 41: 870–878.
[2]. Hormaza, J.I., Dollo, L. and Polito, V.S. (1994). Determination of relatedness and geographical movements of Pistacia vera (Pistachio: Anacardiaceae) germplasm by RAPD analysis. Econ. Bot., 48: 349–358.
[3]. Khatri, B.B. and Shrestha, S.L. (2004). Some local potato cultivars and their reaction to viruses. Nepal Agric. Res. J., 5: 19-21.
[4]. Kimura, M. and Crow, J.F. (1964). The number of alleles that can be maintained in a finite population. Genetics, 49: 725-738.
[5]. Lewontin, R.C. (1972). The apportionment of human diversity. Evol. Biol., 6: 381- 398.
[6]. Moisan-Thiéry, M., Hingrat, Y.L. and Kerlan, M.C. (2001). Potato cultivar identification using molecular markers. Acta Horticulturae, 546: 471-477.
[7]. Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci., USA, 70: 3321- 3323.
[8]. Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89: 583-590.
[9]. Raghunathachari, P., Khanna, V.K., Singh, U.S. and Singh, N.K. (2000). RAPD analysis of genetic variability in Indian scented rice germplasm (Oryza sativa L.). Current Sci., 79: 994-998.
[10]. Rocha, B.H.G., Augustin, E., Pereira, A da S., Fortes, GR de L and Peters, J.A. (2002). Caracterização de cultivares de batataatravés de marcadores moleculares. Revista Científica Rural, 7: 42-51.
[11]. Shrestha, S.K. (1996). Bacterial wilt of potato in Nepal: spread, losses and magnitude of disease. In the Proceedings of Integrated Management of Bacterial Wilt of Potato, Lesson from the hills of Nepal 11-18.
[12]. Weising, K., Nybom, H., Wolff, K. and Kahl, G. (2005). DNA Finger-printing in Plants: Principles, Methods and Applications. CRC Press.
[13]. Wulff, E.G., Torres, S. and Vigil, E.G. (2002). Protocol for DNA extraction from potato tubers. Plant Molecular Biology Reporter, 20: 187a- 187e.
Published
2012-04-01
How to Cite
Gauchan, D., Brinegar, C., Hada, A., & Singh, D. (2012). Molecular Characterization of Nepali Potato Cultivars Using Randomly Amplified Polymorphic DNA (Rapd) Markers. Journal of Advanced Laboratory Research in Biology, 3(2), 72-75. Retrieved from http://e-journal.sospublication.co.in/index.php/jalrb/article/view/102
Section
Articles