Journal of Advanced Laboratory Research in Biology Journal of Advanced Laboratory Research in Biology is a UGC Approved, Open access, Peer-reviewed, Biological Science Journal, published quarterly (January, April, July and October) by the Society of Open Science. Society of Open Science en-US Journal of Advanced Laboratory Research in Biology 0976-7614 The Effect of Using Vitavax Fungicide on Microbial Flora of Peas and Barley Roots and Some Vegetative Characteristics <p>The fungicide Vitavax was used to fumigate both barley and pea seeds at concentrations; 0.1, 0.3 and 0.5mg/g seeds and kept for a period reaching five months. The data obtained indicate that germination percentage and the plant growth characteristics were slightly affected with differences in the two plants under investigation. With regard to microbial content in both rhizosphere and rhizoplan; the total viable count was slightly affected after one month of storage, but the counts remained at a good rate till the 5<sup>th</sup> month of storage. The dominant strains belonged to <em>Bacillus</em>, <em>Pseudomonas</em>, <em>Micrococcus</em>, <em>Sarcina</em> and <em>Actinomyces</em>. The counts of <em>Azotobacter</em> were not affected in a high degree. The fungicide concentration of 0.3mg/g was the best among the treatments with 3 months storage period, for either plant growth parameters or bacterial counts although it reached five months with other treatments.</p> Salem A. Mahfouz Abdel Kader S. El-Zen ##submission.copyrightStatement## 2011-04-01 2011-04-01 2 2 23 29 Testicular and Epididymal Toxicity of Carbaryl in Sprague-Dawley Strain Rats <p>In the past decade, the use of carbamate has become exceedingly popular as that of the organophosphates. There is a growing concern that the exposure to these compounds is a chronic hazard to man and animals. The present study was conducted to investigate the testicular and epididymal toxicity of carbaryl on rats. Male rats of Sprague-Dawley strain were administered orally with carbaryl dissolved in olive oil at 50 - 150mg doses/Kg body weight/day for 30 days. At the end of the treatment, rats were weighed and killed. Body weights remained unchanged at the end of the treatment. The weights of testis and epididymis decreased significantly after the treatment along with significant reductions in epididymal sperm count, viability and sperm motility. Activities of 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase showed a significant decrease along with the reduction in the serum pituitary LH, FSH and testosterone. Carbaryl significantly decreased the activities of epididymal marker enzymes, phosphodiesterase and adenylate cyclase. These findings demonstrate the adverse effect of carbaryl on testicular steroidogenesis and epididymal functions contributing to the decline in the sperm counts of rats.</p> K. C. Chitra E. M. Manogem Y. Vardhanan Shibu C. D. Sebastian K. Jayakumar ##submission.copyrightStatement## 2011-04-01 2011-04-01 2 2 30 34