Journal of Advanced Laboratory Research in Biology http://e-journal.sospublication.co.in/index.php/jalrb Journal of Advanced Laboratory Research in Biology is a UGC Approved, Open access, Peer-reviewed, Biological Science Journal, published quarterly (January, April, July and October) by the Society of Open Science. Society of Open Science en-US Journal of Advanced Laboratory Research in Biology 0976-7614 Control of Ralstonia solanacearum Infection in Tomato, Brinjal and Capsicum by antibiotic sensitivity test http://e-journal.sospublication.co.in/index.php/jalrb/article/view/184 <p><em>Ralstonia solanacearum</em> is one of the important dreaded soil’s borne bacterial phytopathogen which causes enormous losses in the crop plants in tropical, subtropical and temperate region of the world. In India, the disease is highly prevalent and active throughout the year where the soil is acidic in the Eastern Plateau and Hill Region. Once the disease is established in the field, it cannot be controlled by chemical means. Keeping these in view, screening of potential antibiotics for the management of <em>R. solanacearum</em> was done.</p> <p>In the present study, three strains of <em>R. solanacearum</em> were isolated from Brinjal, Tomato and Capsicum. Against these three strains of <em>R. solanacearum</em>, four antibiotics were screened through food poison techniques viz. Kasugamycin, Streptomycin, Ceftriaxone and Gentamicin. The different strains of <em>R. solanacearum</em> and antibiotic sensitivity showed varied response. Among the screened antibiotics, Gentamicin showed strong antibacterial efficacy which inhibited 100% Colony-forming Unit (cfu) at very low concentration (1 ppm) followed by Ceftriaxone which inhibited &gt;50% cfu at 1 ppm against all three strains. However, Streptomycin also showed antibacterial efficacy and inhibited &gt;50% cfu at 3 ppm, but Kasugamycin was found less antibacterial as compared to other tested antibiotics and inhibited &gt;50% cfu at 4 ppm.</p> Rupa Verma Abhijit Dutta Ashok Kumar Choudhary Sudarshan Maurya ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2014-07-01 2014-07-01 5 3 35 40 Regenerative callus induction and biochemical analysis of Stevia rebaudiana Bertoni http://e-journal.sospublication.co.in/index.php/jalrb/article/view/185 <p>Stevia Leaves are the principal source of stevioside, which is estimated to be 100-300 times sweeter than table sugar. Stevioside has clinical significance as they are reported to maintain glucose levels in human blood. Owing to the difficulties in propagation of stevia through seeds and vegetative methods, callus culture has been an efficient alternative for generation of stevioside. The aim of this study is to develop an efficient and standardized protocol for maximum induction and multiplication of callus from a leaf. Callus culture was established from leaves in MS basal media fortified with various combinations (BAP, NAA, 2,4-D, KN, IBA) and concentrations of phytohormones. The best callusing (100%) was recorded in MS media supplemented with (2,4-D 1.0mg/l + NAA 1.0mg/l). The callus was harvested after 4 weeks and screened for the presence of various bioactive compounds. The qualitative results showed that the extracts of callus contained bioactive compounds like flavonoids, glycosides, phenol, tannins, sterols and saponins thereby making callus one of the sources for extraction of various secondary metabolites.</p> Dhurva P. Gauchan Ashna Dhakal Nisha Sharma Sabin Bhandari Elina Maskey Nayan Shrestha Rachita Gautam Sarala Giri Sushma Gurung ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2014-07-01 2014-07-01 5 3 41 45 Marine Sourced Glycosaminoglycans ‘GAGs’ http://e-journal.sospublication.co.in/index.php/jalrb/article/view/186 <p>Globally, there is an uprising interest for availability of tangible, novel, added value, sustainable commercial exploitable therapeutic compounds from naturally derived sources. The paper will highlight bioactive compounds of therapeutic potential from marine sources such as polysaccharides, especially glycosaminoglycans. It is now well documented, and this effort combined with a green technological approach in sustainable natural biodiversity will allow the development of a new generation of therapeutics. Outsourcing for a biocompatible, non-mutational bioactive compound such as GAG’s in a worldwide research and industrial agenda. Hence, the thorough understanding on how the biosynthesis, structure and function of complex glycosaminoglycans will reveal the polysaccharides from the marine environment could provide a valid alternative to traditional polysaccharides such as glycosaminoglycans.</p> Nur Hanim Zainudin K. N.S. Sirajudeen Farid C. Ghazali ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2014-07-01 2014-07-01 5 3 46 53