Comparative Analysis of the Chemical Composition of Juniperus excelsa ssp. polycarpos Bark and Wood Extracts
Keywords:Bark and wood extracts, Chemical composition, Juniperus excelsa ssp. polycarpos, Pimaric acid
In the present study, extracts from the bark and wood of Juniperus excelsa ssp. polycarpos were obtained with acetone solvent. Chemical composition were analyzed and compared by gas chromatography-mass spectrometry (GC-MS). The results showed that the major components identified in the bark acetone extract as trimethylsilyl (TMS) derivatives were β-d-glucofuranose, 1,2,3,5,6-pentakis-O-(TMS) (19.97%), followed by pimaric acid TMS (18.89%), d-mannopyranose,1,2,3,4,6-pentakis-O-(TMS) (13.90%), d-fructose,1,3,4,5,6-pentakis-O-(TMS) (12.37%). The major components identified in the wood acetone extract as trimethylsilyl (TMS) derivatives were pimaric acid TMS (24.56%), followed by α-d-glucopyranoside,1,3,4,6-tetrakis-O-(TMS)-β-d-fructofuranosyl 2,3,4,6-tetrakis-O-(TMS) (21.39%), β-d-galactopyranose,1,2,3,4,6-pentakis-O-(TMS) (12.10%), d-glucose,2,3,4,5,6-pentakis-O-(TMS) (9.97%), trifluoromethyl-bis-(TMS) methyl ketone (9.32%). One of the more important components identified both in the bark and wood extracts was pimaric acid TMS. Cedrol as the essential oil was found in the acetone wood extract (0.72%).
Adams, R.P. (1995). Identification of Essential oil Components by Gas Chromatography/Mass Spectrometry. Allured Publishing Corp., Carol Stream, IL.
Adams, R.P. (2001). Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. 3rd ed., Allured Publishing Corp., Carol Stream, IL.
Angioni, A., Barra, A., Russo, M.T., Coroneo, V., Dessi, S. & Cabras, P. (2003). Chemical composition of the essential oils of Juniperus from ripe and unripe berries and leaves and their antimicrobial activity. J. Agric. Food Chem., 51(10): 3073–3078. https://doi.org/10.1021/jf026203j.
Burits, M., Asres, K. & Bucar, F. (2001). The antioxidant activity of the essential oils of Artemisia afra, Artemisia abyssinica and Juniperus procera. Phytother. Res., 15(2): 103–108. https://doi.org/10.1002/ptr.691.
Filipowicz, N., Kamiński, M., Kurlenda, J., Asztemborska, M. & Ochocka, J.R. (2003). Antibacterial and antifungal activity of juniper berry oil and its selected components. Phytother. Res., 17(3): 227–231. https://doi.org/10.1002/ptr.1110.
Flores, R.M. (2013). Terpene and Terpenoid Emissions and Secondary Organic Aerosol Production. Dissertation, Michigan Technological University. https://doi.org/10.37099/mtu.dc.etds/818.
Hosseinihashemi, S.K., Dadpour, A. & Lashgari, A. (2017). Antioxidant activity and chemical composition of Juniperus excelsa ssp. polycarpos wood extracts. Nat. Prod. Res., 31(6): 681–685. https://doi.org/10.1080/14786419.2016.1209666.
Joseph-Nathan, P., Santillan, R.L. & Gutiérrez, A. (1984). 13C-NMR Study of Cedrol, 6-Isocedrol, and α-Cedrene. J. Nat. Prod., 47(6): 924–933. https://doi.org/10.1021/np50036a003.
Joulain, D. & König, W.A. (1988). The Atlas of Spectral Data of Sesquiterpene Hydrocarbons. E.B. Verlag, Hamburg, Germany.
Lamparsky, D. & Klimes, I. (1985). New results of the analysis of Juniper Berry Oil in view of the Terpenoid Components. Parfüm. Kosmet., 66: 553-558.
Lewinsohn, E., Gijzen, M. & Croteau, R. (1991). Defense mechanisms of conifers: differences in constitutive and wound-induced monoterpene biosynthesis among species. Plant Physiol., 96(1): 44–49. https://doi.org/10.1104/pp.96.1.44.
Loizzo, M.R., Tundis, R., Conforti, F., Saab, A.M., Statti, G.A. & Menichini, F. (2007). Comparative chemical composition, antioxidant and hypoglycaemic activities of Juniperus oxycedrus ssp. oxycedrus L. berry and wood oils from Lebanon. Food Chem., 105(2): 572–578. https://doi.org/10.1016/j.foodchem.2007.04.015.
Okasaka, M., Takaishi, Y., Kashiwada, Y., Kodzhimatov, O.K., Ashurmetov, O., Lin, A.J., Consentino, L.M. & Lee, K.H. (2006). Terpenoids from Juniperus polycarpus var. seravschanica. Phytochemistry, 67(24): 2635–2640. https://doi.org/10.1016/j.phytochem.2006.07.020.
Soković, M., Ristić, M. & Grubišić, D. (2004). Chemical Composition and Antifungal Activity of the Essential Oil from Juniperus excelsa Berries. Pharm. Biol., 42(4-5): 328–331. https://doi.org/10.1080/13880200490511936.
Tunalier, Z., Kirimer, N. & Başer, K.H.C. (2003). Wood Essential Oils of Juniperus foeticissima Willd. Holzforschung, 57(2): 140–144. https://doi.org/10.1515/HF.2003.022.
Yesenofski, J. (1996). Juniper oil distillation and marketing project: western juniper commercialization program. In: The Confederated Tribes of the Warm Springs Reservation of Oregon Business and Economic Development Branch, Final Report Ver. 2.
Zargari, A. (1993). Medicinal Plants. Volume 4. Tehran: Tehran University Press.
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