Effective Biosurfactants Production by Pseudomonas aeruginosa and its Efficacy on Different Oils

  • Sarita Kumari Department of Microbiology, The Oxford College of Science, Bangalore-102, India.
  • K. V. Sekar Department of Microbiology, The Oxford College of Science, Bangalore-102, India.
  • A. Nagasathya P.G. and Research Department of Microbiology, J.J. College of Arts and Science, Pudukkottai- 622404, India.
  • S. Palanivel P.G. and Research Department of Microbiology, J.J. College of Arts and Science, Pudukkottai- 622404, India.
  • Subramanyam Nambaru CAS in Marine Biology, Annamalai University, Parangipettai, Tamil Nadu, India.
Keywords: Biosurfactant production, Pseudomonas aeruginosa, Test efficacy, Rhamnolipid


A rhamnolipid producing bacterium, Pseudomonas aeruginosa was isolated from contaminated soil with oily wastes. The Pseudomonas aeruginosa grown with glucose and corn oil as a carbon source produced bio-surfactant. This biosurfactant was purified by procedures that included chloroform-ethanol extraction and 0.05M bicarbonate treatments. The active compound was identified as rhamnolipid by using thin layer chromatography. The emulsification activity of bio-surfactant, the coconut oil responded better than the olive oil, groundnut oil and sunflower oil and gave a maximum level of 1 cm.


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How to Cite
Kumari, S., Sekar, K., Nagasathya, A., Palanivel, S., & Nambaru, S. (2010). Effective Biosurfactants Production by Pseudomonas aeruginosa and its Efficacy on Different Oils. Journal of Advanced Laboratory Research in Biology, 1(1), 31-34. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/10