Comparative Study for the Effect of Gibberellic acid, Kinetin and Indole-3-acetic acid on Seed Germination performance of Dianthus caryophyllus
Keywords:Carnation, Dianthus caryophyllus, germination percent, plant
Seed germination is the major limiting factor for large-scale production and cultivation of crop species. Such attribute also positively as well as negatively affected by some potent plant growth regulators and other chemical compounds. For this, present experiment was undertaken with an objective to investigate the comparison of the effect of various concentrations of plant growth regulators like Gibberellic Acid (GA3), Kinetin and Indole-3-acetic acid (IAA) on seed germination of Dianthus caryophyllus or Carnation. Dianthus seeds were soaked in different concentrations (0 ppm or control, 10, 20, 30 and 40 ppm) of each of GA3, Kinetin and IAA for 24 h at room temperature (25±2°C). Three replicates of each treatment with ten seeds per replicate were arranged for precise physiological analysis. Significant variation was found in all aspects after analysis of variance (ANOVA) of each mean value. After two weeks of seed soaking, it was noted that germination percentages were significantly accelerated by lower concentrations (10 and 20 ppm) of used hormones. Amongst the three potential growth regulators, 20 ppm was found most effective because it showed highest germination percentage for GA3 (87.46%), Kinetin (78.92%) and IAA (75.35%). A great deal of information relating to seed germination practices shows that these plant growth regulators were efficient to overcoming dormancy leading to rapid seed germination. GA3 was selected as best hormone, in this study, which showed highest seed germination. These results could useful to large-scale cultivation of Dianthus caryophyllus plants for improving its floricultural impact worldwide.
Taiz, L. & Zeiger, E. (2010). Plant Physiology. 5th Edition, Sinauer Associates Inc., Sunderland, 782 p.
Karssen, C.M., Groot, S.P.C. & Koornneef, M. (1987). Hormone mutants and seed dormancy in Arabidopsis and tomato. In: H. Thomas & D. Grierson (eds). Developmental mutants in Higher Plants. SEB Seminar Series 32. Cambridge: Cambridge University Press. pp. 119-133.
Procházka, S., Macháčková, I., Krekule, J. & Šebánek, J. (1998). Fyziologie rostlin (Plant physiology). 1st Edn., Academia, Praha. pp. 484.
Phillips, I.D.J. (1962). Some Interactions of Gibberellic Acid with Naringenin (5,7, 4′-trihydroxy flavanone) in the Control of Dormancy and Growth in Plants. J. Exp. Bot., 13(2): 213–226. https://doi.org/10.1093/jxb/13.2.213.
Wright, S.T.C. (1968). Multiple and sequential roles of plant growth regulators. In: Wightman, F. & G. Setterfield (ed.), Biochemistry and Physiology of Plant Growth Substances. Runge Press, Ottawa. pp. 521-542.
orcoran, M.R. & West, C.A. (1968). Inhibitors from Carob (Ceratonia siliqua L.) I. Nature of the Interaction with Gibberellic Acid on Shoot Growth. Plant Physiol., 43(6): 859–864. https://doi.org/10.1104/pp.43.6.859.
El-Barghathi, M.F. & El-Bakkosh, A. (2005). Effect of some mechanical and chemical pre-treatments on seed germination and seedling growth of Quercus coccifera (Kemes Oaks). Jerash Private University.
Chauhan, J.S., Tomar, Y.K., Singh, N.I., Ali, S. & Debarati, A. (2009). Effect of growth hormones on seed germination and seedling growth of black gram and horse gram. J. Am. Sci., 5(5): 79-84.
Mitra, G.C. (1986). In vitro culture of orchid seeds for obtaining seedlings. In: S.P. Viz (eds), Biology, Conservation and Culture of orchids. East West Press. New Delhi. pp. 401-409.
Hadley, G. (1970). The interaction of kinetin, auxin and other factors in the development of north temperate orchids. New Phytol., 69(2): 549–555. https://doi.org/10.1111/j.1469-8137.1970.tb07607.x.
Mohammed, A.H.M.A. (2007). Physiological Aspects of Mungbean Plant (Vigna radiata L. wilczek) in Response to Salt Stress and Gibberellic Acid Treatment. Res. J. Agric. Biol. Sci., 3(4): 200-213.
Chakrabarti, N. & Mukherji, S. (2003). Effect of Phytohormone Pretreatment on Nitrogen Metabolism in Vigna radiata Under Salt Stress. Biol. Plant., 46(1): 63–66. https://doi.org/10.1023/A:1022358016487.
Haroun, S.A., Badawy, A.H. & Shukry, W.M. (1991). Auxin induced modification of Zea mays and Lupinus termis seedlings exposed to water stress imposed by polyethylene glycol (PEG 6000). Sci. J., 18: 335.
Hoque, M. & Haque, S. (2002). Effects of GA3 and its Mode of Application on Morphology and Yield Parameters of of Mungbean Vigna radiata L.). Pak. J. Biol. Sci., 5: 281-283. https://dx.doi.org/10.3923/pjbs.2002.281.283 .
Aldesuquy, H.S. & Ibrahim, A.H. (2001). Interactive Effect of Seawater and Growth Bioregulators on Water Relations, Abscisic Acid Concentration and Yield of Wheat Plants. J. Agron. Crop Sci., 187(3): 185–193. https://doi.org/10.1046/j.1439-037x.2001.00522.x.
Das Gupta, P., Das, D. & Mukherji, S. (1994). Role of phytohormones in the reversal of stress-induced alteration in growth, turgidity and proline accumulation in mungbean (Vigna radiata L. Wilczek) plants. Ind. Biol., 26: 343-348.
Chakrabarti, N. & Mukherji, S. (2002). Effect of phytohormone pretreatment on metabolic changes in Vigna radiata under salt stress. J. Environ. Biol., 23(3): 295–300.
Roychowdhury, R. & Tah, J. (2011a). Chemical mutagenic action on seed germination and related agrometrical traits in M1 Dianthus generation. Current Bot., 2: 19-23.
Roychowdhury, R. & Tah, J. (2011). Mutation breeding in Dianthus caryophyllus for economic traits. Electronic J. Plant Breed., 2 (2): 282-286.
Roychowdhury, R., Alam, M.J.F., Bishnu, S., Dalal, T. & Tah, J. (2012). Comparative study for effects of chemical mutagenesis on seed germination, survivability and pollen sterility in M1 and M2 generations of Dianthus. Plant Breed. Seed Sci., 65: 29-38.
Kumari, P. & Dahiya, O.S. (2007). Seed germination: optimum temperature, moisture and light. In: S.S. Narwal, B. Politycka & C.L. Goswami (Eds.), Research Methods in Plant Science: Allelopathy, Vol. 5, Plant Physiology. Scientific Publishers, Jodhpur, India.
Panse, V.G. & Sukhatme, P.V. (1967). Statistical Methods for Agricultural Workers. 2nd Edn., ICAR publication, India, New Delhi, pp. 381.
Roychowdhury, R., Tah, J., Dalal, T. & Bandyopadhyay, A. (2011). Selection Response and Correlation Studies for Metrical Traits in Mutant Carnation (Dianthus caryophyllus L.) Genotypes. C. J. Agric. Sci., 5: 06-14.
Ben-Zioni, A., Itai, C. & Vaadia, Y. (1967). Water and salt stresses, kinetin and protein synthesis in tobacco leaves. Plant Physiol., 42(3): 361–365. https://doi.org/10.1104/pp.42.3.361.
Levitt, J. (1980). Responses of plants to environmental stresses. Volume II. Water, radiation, salt, and other stresses. Academic Press.
Harris, D., Joshi, A., Khan, P.A., Gothkar, P. & Sodhi, P.S. (1999). On-farm seed priming in semi-arid agriculture: development and evaluation in maize, rice and chickpea in India using participatory methods. Exp. Agric., 35(1): 15–29. https://doi.org/10.1017/S0014479799001027.
Mohanty, S.K. & Sahoo, N.C. (2000). Effect of soaking period, seed size and growth regulators on imbibitions and germination of seeds of some field crops. Orissa J. Agric. Res., 5 (1-2): 30-35.
Mikulík, J. & Vinter, V. (2002). Evaluation of factors affecting germination of Dianthus superbus L. subsp. Superbus. Biol., 40: 13-18.
Yoshida, I. & Hirasawa, E. (1996). Gibberellin induces endopeptidase activity in detached cotyledons of Pisum sativum. Plant Growth Regul., 19(1): 55–60. https://doi.org/10.1007/BF00024402.
Corcoran, M.R. (1970). Inhibitors from Carob (Ceratonia siliqua L.): II. Effect on Growth Induced by Indoleacetic Acid or Gibberellins A1, A4, A5, and A7. Plant Physiol., 46(4): 531–534. https://doi.org/10.1104/pp.46.4.531.
How to Cite
This work is licensed under a Creative Commons Attribution 4.0 International License.