Isolation, Identification and Seasonal Distribution of Soilborne Fungi in Different Areas of Erbil Governorate

Authors

  • Farid M. Toma Biology Department, College of Science, University of Salahaddin –Erbil, Iraq.
  • Nareen Q. Faqi Abdulla Biology Department, College of Science, University of Salahaddin –Erbil, Iraq.

Keywords:

Soil fungi, Aspergillus sp., Washing method

Abstract

A survey study was undertaken for the isolation and identification of soilborne fungi inhabiting different areas in Erbil, Iraq. A total of 15 soil samples collected seasonally during August 2008-July 2009.

Fungi were isolated from soil during this study's period, by soil dilution plate method on selective medium: Potato Dextrose Agar (PDA), eighty-one different genera of fungi were isolated during the four seasons; (30, 33, 70, and 53) genera isolated in (summer, autumn, winter and spring) respectively, the most frequently isolated fungi during four seasons were Aspergillus sp. (539x103) colony forming units /g.soil, followed by Penicillium sp. (215x103) cfu/g.soil, Rhizopus spp. (115x103) cfu/g.soil, Emericella spp. (109x103) cfu/g.soil, Fusarium spp. (47x103) cfu/g.soil, and Ulocladium botrytis (47x103) cfu/g.soil, while the least frequently isolated fungal genera were Blakeslea tuningtam, Clasterosporium cyperi, Idriella sp., Naranus cryptomeriae and Torula alternata, (1x103) cfu/g.soil, for each one.

Fungi isolated from soil by washing method counted for ninety three species belonging to fifty six genera, among them: Aspergillus sp., Circinella sp., Cunninghamella sp., Mucor spp., Mycelia sterilia, Rhizopus sp.

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References

Abdulkarim, P.M. (2008). Isolation and Identification of fungi from Koya soils and Extraction of Antibiotic from Penicillium Citrinum. M.Sc. Thesis. College of Science, University of Koya.

Ainsworth, G.C., Sparrow, F.K. & Sussman, A.S. (1973). The Fungi. An Advanced Treatise. Vol. IV A: A Taxonomic Review with Keys: Ascomycetes and Fungi Imperfecti. Academic Press, London. pp. 621.

Ali-Shtayeh, M.S. & Jamous, R.M.F. (2000). Keratinophilic fungi and related dermatophytes in polluted soil and water habitats. In: Kushwaha, R.K.S. & Guarro, J. (eds), Biology of Dermatophytes and Other Keratinophilic Fungi. Bilbao: Revista Iberoamericana de Micología. pp. 51-59.

Arif, I.A. & Hashem, R. (1998). Soil analysis and mycoflora of Jizan city, Saudi Arabia. Phyton, 62(1-2): 109–113.

Aşkun, T. (2007). Comparison of two medium according to mould enumeration and recovered species from wheat and feed. J. Appl. Biol. Sci., 1(3): 37-42.

Azaz, A.D. (2003). Isolation and Identification of Soilborne Fungi in Fields Irrigated by GAP in Harran Plain Using Two Isolation Methods. Turk. J. Bot., 27(2): 83–92.

Azaz, A.D. & Pekel, O. (2002). Comparison of Soil Fungi Flora in Burnt and Unburnt Forest Soils in the Vicinity of Kargıcak (Alanya, Turkey). Turk. J. Bot., 26(6): 409–416.

Barnett, H.L. (1969). Illustrated Genera of Imperfect Fungi. 2nd ed. Burgess Publishing Company. pp. 203.

Barnett, H.L. & Hunter, B.B. (1972). Illustrated Genera of Imperfect Fungi. 3rd ed. Minneapolis, Burgess Publishing Company. pp. 241.

Basu, P.K. (1980). Production of chlamydospores of Phytophthora megasperma and their possible role in primary infection and survival in soil. Can. J. Plant Pathol., 2(2): 70–75. https://doi.org/10.1080/07060668009501441.

Beneke, E.S., Rippon, J.W. & Rogers, A.L. (1984). Human Mycoses, A Scope Monograph. 8th ed. The Upjohn Company, Kalamazoo, MI.

Benson, H.J. (2002). Microbiological Applications: Laboratory Manual in general microbiology. 8th ed. McGraw Hill, New York. pp. 478.

Bessey, E.A. (1968). Morphology and Taxonomy of Fungi. Hafner Publishing Company. pp. 789.

Brady, N.C. & Weil, R.R. (2002). The Nature and Properties of Soil. 13th ed. Prentice Hall, NJ. pp. 960.

Chaturvedi, V. & Ren, P. (2007). Mycology Laboratory. Proficiency Testing Program Coordinator. Wadsworth Center. New York State Department of Health. pp. 52.

Djamel, C., Ali, T. & Nelly, C. (2009). Acid protease production by isolated species of Penicillium. Eur. J. Sci. Res., 25(3): 469-477.

El-Amin, A.N. & Saadabi, A.M. (2007). Contribution to the Knowledge of Soil Fungi in Sudan Rhizosphere Mycoflora of Sugarcane at Kenana Sugar Estate. Int. J. Botany, 3: 97-102. https://dx.doi.org/10.3923/ijb.2007.97.102.

Ellis, M.B. (1971). Dematiaceous hyphomycetes. Commonwealth Mycological Institute Kew, London UK. pp. 608.

Frey, D., Oldfield, R.J. & Bridger, R.C. (1979). A Colour Atlas of Pathogenic Fungi. Wolfe Medical Publications. London. pp. 168.

Gams, W., Van der Aa, H.A., van der Plaats-Niterink, A.J., Samson, R.A. & Stalpers, J.A. (1987). CBS Course of Mycology. 3rd ed., Centraalbureau voor Schimmelcultures, Baarn, The Netherlands. p. 136.

Gilman, J.C. (1966). A Manual of Soil Fungi. 2nd ed. Iowa State University Press, Ames, Iowa. pp. 450.

Guarro, J., Gené, J. & Stchigel, A.M. (1999). Developments in Fungal Taxonomy. Clin. Microbiol. Rev., 12(3): 454–500. https://doi.org/10.1128/CMR.12.3.454.

Hashem, A.R. (1991). Studies on the fungal flora of Saudi Arabian soil. Cryptogam Botany, 2(3): 179-182.

Howard, D.H. (2002). Pathogenic fungi in humans and animals. 2nd ed. Marcel Dekker, Inc., New York. pp. 790.

Ingham, E.R. (2000). Soil Fungi. Chapter 4. Soil Biology Primer. Rev. ed., Ankeny, Iowa: Soil and Water Conservation Society. http://www.nrcs.usda.gov/wps/portal/nrcs/main/soils/health/biology

Johnston, A. & Booth C. (1983). Plant Pathologist's Pocketbook. 2nd Ed., Commonwealth Mycological Institute, Kew. pp. 439.

Larone, D.H. (1995). Medically important fungi: A guide to identification. 3rd ed., ASM press, Washington DC. pp. 274.

McClenny, N. (2007). An Unusual Aspergillus Species at a Major Cancer Center: Implications for the Clinical Laboratory San Francisco State University, San Francisco, CA Course DL-977 1.0 CE/Contact Hour Level: Beginning to Intermediate. MPA, MT (ASCP).

Moubasher, A.H. (1993). Soil fungi in Qatar and other Arab countries. Center for Scientific and Applied Research, University of Qatar. pp. 566.

Onuorah, O.M.O. (1982). Studies on the elicitation of Lucerne of Medicago sativa L. phytoalexins by Verticillium spp. Ph.D. Thesis. University of Wales.

Pitt, J.I. & Hocking, A.D. (1997). Fungi and Food Spoilage. 2nd ed. Blackie Academic & Professional, London. pp. 593.

Pornsuriya, C., Lin, F.C., Kanokmedhakul, S. & Soytong, K. (2008). New record of Chaetomium species isolated from soil under pineapple plantation in Thailand. J. Agric. Technol., 4(2): 91-103.

Rajankar P., Tambekar D. & Wate S. (2007). Study of phosphate solubilization efficiencies of fungi and bacteria isolated from saline belt of Purna river basin. Res. J. Agric. Biol. Sci., 3: 701–703.

Rane, G. & Gandhe, R.V. (2006). Seasonal distribution of soil fungi from forest soils of Jalgaon district, Maharashtra. Zoos' Print Journal, 21(9): 2407–2409.

Rønhede, S., Jensen, B., Rosendahl, S., Kragelund, B.B., Juhler, R.K. & Aamand, J. (2005). Hydroxylation of the Herbicide Isoproturon by Fungi Isolated from Agricultural Soil. Appl. Environ. Microbiol., 71(12): 7927–7932. https://doi.org/10.1128/AEM.71.12.7927-7932.2005.

Rusuku, G., Buruchara, R.A., Gatabazi, M. & Pastor-Corrales, M.A. (2007). Occurrence and Distribution in Rwanda of Soilborne Fungi Pathogenic to the Common Bean. Plant Dis., 81(5): 445-449. https://doi.org/10.1094/PDIS.1997.81.5.445.

Saadoun, I., Mohammad, M.J., Hameed, K.M. & Shawaqfah, M. (2008). Microbial populations of crude oil spill polluted soils at the Jordan-Iraq desert (the Badia region). Braz. J. Microbiol., 39(3): 453–456. https://doi.org/10.1590/S1517-83822008000300008.

Samson, R.A., Hoekstra, E.S. & Van Oorschot, C.A.N. (1981). Introduction to food-borne fungi. Institute of the Royal Netherlands Academy of Arts and Sciences. pp. 247.

Scholtz, O.F. (2006). Citric acid induced phytoextraction of heavy metals from uranium contaminated soils. M.Sc. Thesis. Department of Plant Sciences. University of the Free State.

Sylvia, D.M., Fuhrmann. J.J., Hartel, P.G. & Zuberer, D.A. (2005). Principles and Applications of Soil Microbiology. 2nd ed., Pearson Education, Upper Saddle River, NJ.

Tugel, A.J., Lewandowski, A.M. & Happe-vonArb, D. eds. (2000). Soil Biology Primer. Ankeny, IA: Soil and Water Conservation Society.

İlhan, S., Demi̇rel, R., Asan, A., Bayçu, C. & Kinaci, E. (2006). Colonial and Morphological Characteristics of Some Microfungal Species Isolated from Agricultural Soils in Eskişehir Province (Turkey). Turk. J. Bot., 30(2): 95–104.

Uztan, A.H., Ate, M. & Abaci, O. (2006). Emericella quadrilineata (Thom and Raper) CR Benjamin (Ascomycetes): First Reports from Turkiye JFS. EUFF (Turkey), 29: 33-40.

Watanabe, T. (2002). Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species. 2nd ed., CRC Press, Boca Raton. pp. 484. https://doi.org/10.1201/9781420040821

Zydlik, Z., Rutkowski, K. & Pacholak, E. (2006). Effect of soil fatigue prevention methods on microbiological soil status in replanted apple tree orchard. Part iii. Number of fungi and actinomycetes, Electron. J. Pol. Agric. Univ., 9(4): 58.

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Published

01-10-2012

How to Cite

Toma, F. M., & Abdulla, N. Q. F. (2012). Isolation, Identification and Seasonal Distribution of Soilborne Fungi in Different Areas of Erbil Governorate. Journal of Advanced Laboratory Research in Biology, 3(4), 246–255. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/134

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