Isolation, Purification and Characterization of Oxygen Insensitive Azoreductase from Pseudomonas aeruginosa and Biodegradation of Azo Dye - Methyl Red
Keywords:Pseudomonas aeruginosa, Decolorization, Azoreductase, Methyl red
A Pseudomonas aeruginosa was isolated from water sample from Industrial effluent and was tested for decolorization activity against commercially important dye of Methyl red. Percentage dye degradation by the isolated Pseudomonas aeruginosa was found to be 90%. The enzyme involved in degradation azoreductase was assayed and purified by anion-exchange chromatography. Total activity of the purified enzyme was 22.5U/mg. The enzyme gave a single band in the SDS–PAGE with a molecular weight of 29 kDa (approximately). The maximal azoreductase activity was observed at pH 7.0 and at 37°C. This activity was NADH dependent. Several metal ions inhibited the purified enzyme including Fe2+ and Hg2+.
Carliell, C.M., Barclay, S.J., Naidoo, N., Buckley, C.A., Mulholland, D.A. & Senior, E. (1995). Microbial decolorization of a reactive azo dye under anaerobic conditions. Water SA, 21: 61–69.
Chang, J.-S. & Lin, C.-Y. (2001). Decolorization kinetics of a recombinant Escherichia coli strain harboring azo-dye-decolorizing determinants from Rhodococcus sp. Biotechnol. Lett., 23(8): 631–636. https://doi.org/10.1023/A:1010306114286.
Chen, H. (2006). Recent advances in azo dye degrading enzyme research. Curr. Protein Pept. Sci., 7(2): 101–111. https://doi.org/10.2174/138920306776359786.
Churchley, J.H. (1994). Removal of dyewaste colour from sewage effluent – the use of a full scale ozone plant. Water Sci. Technol., 30(3): 275–284. https://doi.org/10.2166/wst.1994.0120.
Clark, J.M. & Switzer, R.L. (1977). Experimental Biochemistry. 2nd edition, W.H. Freeman and Company, San Francisco. pp. 82–83.
Heiss, G.S., Gowan, B. & Dabbs, E.R. (1992). Cloning of DNA from a Rhodococcus strain conferring the ability to decolorize sulfonated azo dyes. FEMS Microbiol. Lett., 99(2-3): 221–226. https://doi.org/10.1111/j.1574-6968.1992.tb05571.x.
Kulla, H.G., Klausener, F., Meyer, U., Lüdeke, B. & Leisinger, T. (1983). Interference of aromatic sulfo groups in the microbial degradation of the azo dyes Orange I and Orange II. Arch. Microbiol., 135(1): 1–7. https://doi.org/10.1007/BF00419473.
Rashamuse, K.J. (2003). The bioaccumulation of platinum (IV) from aqueous solutions using sulphate reducing bacteria: role of a hydrogenase enzyme. MSc Thesis, Rhodes University, Grahamstown, South Africa. 89 p.
Sani, R.K. & Banerjee, U.C. (1999). Decolorization of triphenylmethane dyes and textile and dye-stuff effluent by Kurthia sp. Enzyme Microb. Technol., 24(7): 433–437. https://doi.org/10.1016/S0141-0229(98)00159-8.
Sharma, S., Munjal, A., Gupta, S. & Khan, A. (2011). Kinetic characterization of azoreductase enzyme isolated from Xanthomonas campestris MTCC 10,108. J. Pharm. Res., 4: 2648-2650.
van der Zee, F.P. & Villaverde, S. (2005). Combined anaerobic–aerobic treatment of azo dyes—A short review of bioreactor studies. Water Res., 39(8): 1425–1440. https://doi.org/10.1016/j.watres.2005.03.007.
Zehnder, A.J.B. (1988). Biology of anaerobic microorganisms. 1st Ed., John Wiley & Sons, pp. 471-586.
Zhou, W. & Zimmermann, W. (1993). Decolorization of industrial effluents containing reactive dyes by actinomycetes. FEMS Microbiol. Lett., 107(2-3): 157–161. https://doi.org/10.1111/j.1574-6968.1993.tb06023.x.
Zimmermann, T., Kulla, H.G. & Leisinger, T. (1982). Properties of purified Orange II azoreductase, the enzyme initiating azo dye degradation by Pseudomonas KF46. Eur. J. Biochem., 129(1): 197–203. https://doi.org/10.1111/j.1432-1033.1982.tb07040.x.
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