Effect of Mercuric Chloride on Hepatic Phosphatases and Transaminases in Albino Rat

Authors

  • K. Mahour Toxicology Laboratory, Department of Zoology, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University, Agra-282002, India.
  • P. N. Saxena Toxicology Laboratory, Department of Zoology, School of Life Sciences, Khandari Campus, Dr. B. R. Ambedkar University, Agra-282002, India.

Keywords:

AST, ALT, ALP, ACP, Rattus norvegicus

Abstract

Mercuric chloride is a serious health hazard and produces various disorders. However, phosphatases and transaminases are marker enzymes of hepatic toxicity. Twenty four adult albino rats have taken and divided into 4 groups. Group one for acute study, while three for subacute studies with 3 rats in each. Control was also taken with similar references. Mercuric chloride gave orally administered (LD50=9.26mg/kg b.w.) by gavage tube with distilled water. Rats were autopsized at predetermined time interval to assess hepatic toxicity. Phosphatases include alkaline phosphatase and acid phosphatase while transaminases include alanine transaminase and aspartate aminotransferase. Results revealed that ALP and ACP were significantly increased after acute and subacute treatment due to the destruction of the cell membrane of lysosomes. However, AST and ALT were also increased significantly due to toxic effect of mercuric chloride on hepatic cells. Hence, the present study demonstrates that mercuric chloride produces hepatic toxicity in the form of elevation of phosphatases and transaminases enzyme level.

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References

Berlin, M. (1986). Mercury. In: Friberg, L., Nordberg. G.F. & Vouk, V.B. (eds.). Handbook on the toxicology of metals, Vol. II, Elsevier Science, Amsterdam. pp. 387-445.

Hijova, E., Nistiar, F. & Sipulova, A. (2005). Changes in ascorbic acid and malondialdehyde in rats after exposure to mercury. Bratisl. Lek. Listy., 106(8-9): 248–251.

Clausen, J. (1993). Mercury and multiple sclerosis. Acta Neurol. Scand., 87(6): 461–464. https://doi.org/10.1111/j.1600-0404.1993.tb04137.x.

Hua, M.S., Huang, C.C. & Yang, Y.J. (1996). Chronic elemental mercury intoxication: neuropsychological follow-up case study. Brain Inj., 10(5): 377–384. https://doi.org/10.1080/026990596124386.

Langauer-Lewowicka, H. & Zajac-Nedza, M. (1997). Changes in the nervous system due to occupational metallic mercury poisoning. Neurol. Neurochir. Pol., 31(5): 905–913.

Deleu, D., Hanssens, Y. al-Salmy, H.S. & Hastie, I. (1998). Peripheral polyneuropathy due to chronic use of topical ammoniated mercury. J. Toxicol. Clin. Toxicol., 36(3): 233–237. https://doi.org/10.3109/15563659809028945.

Gassó, S., Suñol, C., Sanfeliu, C., Rodrı́guez-Farré, E. & Cristòfol, R.M. (2000). Pharmacological characterization of the effects of methylmercury and mercuric chloride on spontaneous noradrenaline release from rat hippocampal slices. Life Sci., 67(10): 1219–1231. https://doi.org/10.1016/S0024-3205(00)00715-3.

Saxena, P.N. & Mahour, K. (2006). Haematological alteration followed by mercuric chloride intoxication in albino rat. Indian J. Environ. Toxicol., 16(1): 23-26.

Mahour, K. & Saxena, P.N. (2009). Assessment of haematotoxic potential of mercuric chloride in rat. J. Environ. Biol., 30(5 Suppl): 927–928.

Goyer, R.A. (1991). Toxic effects of metals. In: Amdur, M.O., Doull, J. & Klaassen, C.D. (eds.), Casarett and Doull’s toxicology: The basic science of poisons. New York: Pergamon Press, pp. 629-681.

Finney, D.J. (1971). Probit Analysis. 3rd edn. Cambridge: Cambridge University Press.

NIH (1985). Guide for the care and use of Laboratory Animals. NIH Publication No 85-23, Bethesda, Md.: National Institutes of Health.

Kind, P.R. & King, E.J. (1954). Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J. Clin. Pathol., 7(4): 322–326. https://doi.org/10.1136/jcp.7.4.322.

Reitman, S. & Frankel, S. (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol., 28(1): 56–63. https://doi.org/10.1093/ajcp/28.1.56.

Fisher, R.A. (1954). Statistical methods for research workers. 12th edn., Oliver and Boyd, Edinburgh. pp. 365.

Mitra, S. & Sur, R.K. (1997). Hepatoprotection with Glycosmis pentaphylla (Retz). Indian J. Exp. Biol., 35(12): 1306–1309.

Janbaz, K.H. & Gilani, A.H. (2000). Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents. Fitoterapia, 71(1): 25–33. https://doi.org/10.1016/s0367-326x(99)00098-2.

Hukkeri, V.I., Jaiprakash, B., Lavhale, M.S., Karadi, R.V. & Kuppast, I.J. (2003). Hepatoprotective Activity of Ailanthus Excelsa Roxb. Leaf Extract on Experimental Liver Damage in Rats. Indian J. Pharm. Educ., 37(2): 105-106.

Nair, P.S. (2006). Protective Effect of Tefroli - a polyherbal mixture (Tonic) on cadmium chloride induced hepatotoxic rats. Phcog. Mag., 2(6): 112-118.

Mehra, M. & Kanwar, K.C. (1986). Enzyme changes in the brain, liver and kidney following repeated administration of mercuric chloride. J. Environ. Pathol. Toxicol. Oncol., 7(1-2): 65–71.

Dikshith, T.S. & Raizada, R.B. (1983). Response of carbon tetrachloride pretreated rats to endosulfan, carbaryl and phosphamidon. Ind. Health., 21(4): 263–271. https://doi.org/10.2486/indhealth.21.263.

Johri, S., Shrivastava, S., Sharma, P. & Shukla, S. (2004). Analysis of time-dependent recovery from beryllium toxicity following chelation therapy and antioxidant supplementation. Indian J. Exp. Biol., 42(8): 798–802.

Abou-Seif, M.A., El-Naggar, M.M., El-Far, M., Ramadan, M. & Salah, N. (2003). Prevention of biochemical changes in gamma-irradiated rats by some metal complexes. Clin. Chem. Lab. Med., 41(7): 926–933. https://doi.org/10.1515/CCLM.2003.141.

Moss, D.W., Henderson, A.R. & Kochmar, J.F. (1986). Enzymes; Principles of diagnostic enzymology and the aminotransferases. In: Tietz, N.W. (Ed.), Textbook of Clinical Chemistry, Saunders, Philadelphia, pp. 663-678.

Manjusha, M, Patil, K.M., Zambare, G.N., Khandelwal, K.R. & Bodhankar, S.L. (2004). Hepatoprotective activity of aqueous extract of leaves of Feronia elephantum correa against thioacetamide and allyl alcohol intoxication in rats. Toxicol. Int., 11(2): 69-74.

Adolph, L. & Lorenz, R. (1978). Enzymdiagnostik bei Herz-, Leber- und Pankreaserkrankungen (German Edition). 1st edition, S. Karger.

Biswas, S.J. & Khuda-Bukhsh, A.R. (2004). Evaluation of protective potentials of a potentized homeopathic drug, Chelidonium majus, during azo dye induced hepatocarcinogenesis in mice. Indian J. Exp. Biol., 42(7): 698–714.

Deshpande, U.R., Gadre, S.G., Raste, A.S., Pillai, D., Bhide, S.V. & Samuel, A.M. (1998). Protective effect of turmeric (Curcuma longa L.) extract on carbon tetrachloride-induced liver damage in rats. Indian J. Exp. Biol., 36: 573-577.

Götz, W. (1980). Diagnostik von Lebererkrankungen. Darmstadt: Giebeler, pp. 85-91.

Janbaz, K.H., Saeed, S.A. & Gilani, A.H. (2003). Hepatoprotective Effect of Thymol on Chemical-induced Hepatotoxicity in Rodents. Pak. J. Biol. Sci., 6(5): 448-451.

Kumar, M., Sharma, M.K. & Kumar, A. (2005). Spirulina fusiformis: A Food Supplement against Mercury Induced Hepatic Toxicity. J. Health Sci., 51(4): 424–430. https://doi.org/10.1248/jhs.51.424.

Rathore, H.S. & Varghese. J. (1994a). Effect of mercuric chloride on the survival, food intake, body weight, histological and haematological changes in mice and their presentation with Liv-52. Ind. J. Occupl. Hlt., 37(2): 42-54.

Saxena, P.N. & Mahour, K. (2006). Analysis of hepatoprotection by Panax ginseng following mercuric chloride intoxication in albino rat. In: Proceedings of the 9th International Ginseng Symposium. Korean Society of Ginseng, Korea.

Zaman, R.U. & Ahmad, M. (2004). Evaluation of Hepatoprotective Effects of Raphanas sativus L. J. Biol. Sci., 4: 463-469. https://dx.doi.org/10.3923/jbs.2004.463.469.

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Published

01-10-2012

How to Cite

Mahour, K., & Saxena, P. N. (2012). Effect of Mercuric Chloride on Hepatic Phosphatases and Transaminases in Albino Rat. Journal of Advanced Laboratory Research in Biology, 3(4), 319–322. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/151

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