Prevalence of Cryptosporidium and Giardia lamblia in Water Samples from Jeddah and Makkah Cities

Authors

  • Haytham Ahmed Zakai Faculty of Applied Medical Sciences, King Abdul-Aziz University, P.O. Box 80324, Jeddah 21589, Kingdom of Saudi Arabia.
  • Hanadi Ibrahim Barnawi Faculty of Applied Medical Sciences, King Abdul-Aziz University, P.O. Box 80324, Jeddah 21589, Kingdom of Saudi Arabia.

Keywords:

Cryptosporidium, Giardia, Water contamination, Jeddah, Makkah

Abstract

Water contamination by Giardia lamblia and Cryptosporidium is one of the causes of diarrhoea throughout the world.  A total of 161 and 84 samples were collected from Jeddah and Makkah cities, respectively.  Each sample was concentrated by double centrifugation and the sediment was examined as a wet smear and after staining with Trichrome and Kinyoun stains.  The results showed that 56 (35%) and 1 (0.62 %) samples of Jeddah were positive for the oocyst of Cryptosporidium and cyst of Giardia, whereas only 21 (25%) and 2 (2.4 %) samples of Makkah showed positivity for oocysts and cyst of these parasites. Overall Cryptosporidium contamination in bottled water and water from filling stations was 6.8% and 17.4%, respectively. Maximum contamination for Cryptosporidium was recorded in tap water which was 51% and 25% in Jeddah and Makkah, respectively.

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References

[1]. Ryu, H., Alum, A., Mena, K.D., Abbaszadegan, M. (2007). Assessment of the risk of infection by Cryptosporidium and Giardia in non-potable reclaimed water. Water Sci. Technol., 55(1-2): 283-290.
[2]. Robertson, L.J., Forberg, T., Gjerde, B.K. (2008). Giardia cysts in sewage influent in Bergen, Norway 15-23 months after an extensive waterborne outbreak of giardiasis. J. Appl. Microbiol., 104(4): 1147-1152.
[3]. Tzipori, S. (1983). Cryptosporidiosis in animals and humans. Microbiol. Rev., 47(1): 84-96.
[4]. Forgacs, P., Tarshis, A., Ma, P., Federman, M., Mele, L., Silverman, M.L., Shea, J.A. (1983). Intestinal and Bronchial Cryptosporidiosis in an Immunodeficient Homosexual Man. Ann. Intern. Med., 99(6): 793-794.
[5]. Ma, P., Villanueva, T.G., Kaufman, D., Gillooley, J.F. (1984). Respiratory cryptosporidiosis in the acquired immune deficiency syndrome. Use of modified cold Kinyoun and Hemacolor stains for rapid diagnoses. JAMA, 252(10): 1298-1301.
[6]. Fayer, R., Ungar, B.L.P. (1986). Cryptosporidium spp. and cryptosporidiosis. Microbiol. Rev., 50(4): 458-483.
[7]. Hunter, P.R., Nichols, G. (2002). Epidemiology and clinical features of Cryptosporidium infection in immunocompromised patients. Clin. Microbiol. Rev., 15(1): 145-54.
[8]. Raccurt, C.P., Brasseur, P., Verdier, R.I., Li, X., Eyma, E., Stockman, C.P., Agnamey, P., Guyot, K., Totet, A., Liautaud, B., Nevez, G., Dei-Cas, E., Pape, J.W. (2006). Human cryptosporidiosis and Cryptosporidium spp. in Haiti. Trop. Med. Int. Health, 11(6): 929-934.
[9]. Sanad, M.M., Al-Malki, J.S. (2007). Cryptosporidiosis among immunocompromised patients in Saudi Arabia. J. Egypt Soc. Parasitol., 37(2 suppl.): 765-774.
[10]. Ajjampur, S.S., Sankaran, P., Kang, G. (2008). Cryptosporidium species in HIV-infected individuals in India: an overview. Natl. Med. J. India, 21(4): 178-84.
[11]. Nahrevanian, H., Assmar, M. (2008). Cryptosporidiosis in immunocompromised patients in the Islamic Republic of Iran. J. Microbiol. Immunol. Infect., 41(1): 74-77.
[12]. Eisenstein, L., Bodager, D., Ginzl, D. (2006). Outbreak of giardiasis and cryptosporidiosis associated with a neighborhood interactive water fountain-Florida, 2006. J. Environ. Health, 71(3): 18-22.
[13]. Nishi, L., Baesso, M.L., Santana, R.G., Fregadolli, P., Falavigna, D.L., Falavigna-Guilherme, A.L. (2009). Investigation of Cryptosporidium spp. and Giardia spp. in a public water-treatment system. Zoonoses Public Health, 56(5): 221-228.
[14]. Daly, E.R., Roy, S.J., Blaney, D.D., Manning, J.S., Hill, V.R., Xiao, L., Stull, J.W. (2010). Outbreak of giardiasis associated with a community drinking-water source. Epidemiol. Infect., 138(4): 491-500.
[15]. Al-Mohammed, H.I. (2011). Genotypes of Giardia intestinalis clinical isolates of gastrointestinal symptomatic and asymptomatic Saudi children. Parasitol. Res., 108(6): 1375-1381.
[16]. Garcia, L.S. (2001). Modified Kinyoun’s Acid-Fast stain (cold). Diagnostic Medical Parasitology, 3: 723.
[17]. Spencer, E.M., Monroe, L.S. (2008). SDL stain quick Trichrome stain system. Markell, A.K., J. Parasit., 42: 47.
[18]. Chalmers, R.M., Campbell, B.M., Crouch, N., Charlett, A. and Davies, A.P. (2011). Comparison of diagnostic sensitivity and specificity of seven Cryptosporidium assay used in the UK. J. Med. Microbiol., 60(11): 1598-1604.
[19]. Magi, B., Canocchi, V., Tordini, G., Cellesi, C., Barberi, A. (2006). Cryptosporidium infection: diagnostic techniques. Parasitol. Res., 98(2): 150-152.
[20]. Rigo, C.R., Franco, R.M. (2002). Comparison between the modified Ziehl-Neelsen and Acid-Fast-Trichrome methods for fecal screening of Cryptosporidium parvum and Isospora belli. Rev. Soc. Bras. Med. Trop., 35(3): 209-214.
[21]. Tee, G.H., Moody, A.H., Cooke, A.H., Chiodini, P.L. (1993). Comparison of techniques for detecting antigens of Giardia lamblia and Cryptosporidium parvum in faeces. J. Clin. Pathol., 46(6): 555-558.
[22]. Wallis, P.M., Erlandsen, S.L., Isaac-Renton, J.L., Olson, M.E., Robertson, W.J., Van Keulen, H. (1996). Prevalence of Giardia cysts and Cryptosporidium oocysts and characterization of Giardia spp. isolated from drinking water in Canada. Appl. Environ. Microbiol., 62(8): 2789-2797.
[23]. Carmena, D., Aguinagalde, X., Zigorraga, C., Fernández-Crespo, J.C., Ocio, J.A. (2007). Presence of Giardia cysts and Cryptosporidium oocysts in drinking water supplies in northern Spain. J. Appl. Microbiol., 102(3): 619-629.
[24]. Al-Braiken, F.A., Amin, A., Beeching, N.J., Hommel, M., Hart, C.A. (2003). Detection of Cryptosporidium amongst diarrhoeic and asymptomatic children in Jeddah, Saudi Arabia. Ann. Trop. Med. Parasitol., 97(5): 505-510.
[25]. Areeshi, M.Y., Beeching, N.J., Hart, C.A. (2007). Cryptosporidiosis in Saudi Arabia and neighboring countries. Ann. Saudi Med., 27(5): 325-332.
[26]. Iqbal, J., Khalid, N., Hira, P.R. (2011). Cryptosporidiosis in Kuwaiti children: association of clinical characteristics with Cryptosporidium species and subtypes. J. Med. Microbiol., 60(5): 647-652.
[27]. Kasim, A.A., Elhelu, M.A. (1983). Giardiasis in Saudi Arabia. Acta. Trop., 40(2): 155-158.
[28]. Khan, Z.H., Namnyak, S.S., Al Jama, A.A., Madan, I. (1988). Prevalence of cryptosporidiosis in Dammam and Alkhobar, Saudi Arabia. Ann. Trop. Paediatr., 8(3): 170-172.

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Published

01-01-2014

How to Cite

Zakai, H. A., & Barnawi, H. I. (2014). Prevalence of Cryptosporidium and Giardia lamblia in Water Samples from Jeddah and Makkah Cities. Journal of Advanced Laboratory Research in Biology, 5(1), 12–17. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/179

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