Comparative In silico Analysis of Hypertrophic Cardiomyopathy Heart in Human and Normal Chicken Heart
Keywords:Hypertrophic Cardiomyopathy, Beta-myosin Heavy Chain, CLUSTALW, Phylogenetic tree
Hypertrophic Cardiomyopathy (HCM) is an incurable disease, which causes excessive thickening of the myocardium. HCM is caused by abnormalities in genes which code for the proteins responsible for contraction of the heart. The anatomical resemblances between the Chicken heart and HCM heart are thickening of the muscle of the left ventricle, localized ring- like thickening of muscle under the aortic valve, and poorly formed anterior ventricular groove, and left ventricle projecting beyond the right ventricle. The mutations for the genes are retrieved from the databases: Human Genome Mutation Database, Familial Hypertrophic Mutation Database and Cardiogenomics databases. Since the Beta-myosin Heavy Chain (MYH7) is more predominant in causing HCM, that gene alone is taken and compared with normal chicken heart myh7 gene. The amino acids were aligned by CLUSTAL W and they were found to have sequence homology of 85%. The phylogenetic tree was constructed by using PHYLIP to study evolutionary linkage. According to the results, it can be presumed that the chicken normal gene, during the evolutionary process have undergone many modifications and evolved as a complicated and advanced human heart.
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