In vitro Propagation and Conservation of Inula racemosa Hook. F. an Endangered Medicinal Plant of Temperate Origin
Keywords:Vitrification, Inula racemosa, Micropropagation
Inula racemosa is an endangered medicinal plant. It is commonly known as Pushkarmool, Pushkar and Manu. The great sage Charaka has characterized it as Hikka magrahana (stops hiccups) and Savasahara (helpful in asthma). Also, he has cited it as the best medicament for pleurisy along with cough and asthma. Due to the fragile nature of its habitat and exploitation due to its commercial medicinal properties, the species are facing the onslaught of indiscriminate over-exploitation. So far, this plant has not got the required attention from researchers, hence, except for a few efforts, not much work has been done for its cultivation and conservation. Plant tissue culture offers an attractive and quick method for its multiplication and further conservation. In the present investigation, effective procedures for micropropagation and in vitro conservation by vitrification were developed. In vitro propagation using aseptically grown seedlings and in vitro conservation via vitrification were standardized. The in vitro conserved material could be retrieved and multiplied normally on Murashige and Skoog medium fortified with 1.00 mg l-1BA (benzyl adenine) which has been recorded as the best performing medium for in vitro shoot multiplication. The conserved shoots showed normal in vitro propagation and after retrieval from vitrification, platelets were hardened and successfully established in the experimental fields under Nauni (Solan, HP) conditions at an elevation of around 1275 meters above mean sea level.
Alagumanian, S., Perumal, S.V., Balachander, R., Rameshkanan, R. & Rao, M.V. (2004). Plant regeneration from leaf and stem explants of Solanum trilobatum L. Current Science, 86(11): 1478-1480.
Anonymous (1998). Threatened Medicinal plants of Himalaya A checklist. CIMAP, Workshop, Lucknow.
Bobrowski, V.L., Paulo, M.F. & Peters, J.A. (1996). Micropropagation of blackberries (Rubus species) cultivars. Rev. Bras. de Agro., 2(1): 17-20.
Dhaka, N. & Kothari, S.L. (2005). Micropropagation of Eclipta alba (L.) Hassk – an important medicinal plant. In vitro Cellular and Developmental Biology Plant, 43: 658-661.
Dhar, U. and Joshi, M. (2005). Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Asteraceae: effect of explant type, age and plant growth regulators. Cell Biology and Morphogenesis, 24: 195-200.
Eellarova, E. & Kimakova, K. (1999). Morphoregulatory effects of plant growth regulators on Hypericum perforatum L. seedlings. Acta Biotechnology, 19: 163-169.
Hitmi, A., Barthomeuf, C. & Sallonon, H. (2000). Cryopreservation of Chrysanthemum cinerariifolium shoot tips. Journal of Plant Physiology, 156: 408–412.
Joshi, I., Bisht, P., Sharma, V.K. & Uniyal, D.P. (2003). In vitro clonal propagation of mature Eucalyptus. Silvae Genetica, 52(3-4): 110-113.
Kaloo, A.Z. & Shah, A.M. (1997). Plant regeneration from shoot apical tips of Inula racemosa - a threatened medicinal plant species. Oriental Science, 2: 17-22.
Kaur, R., Sadiq, M., Kumar, V., Mahajan, R., Saxena, B. & Sharma, D.R. (2007). In vitro propagation and conservation of Gentiana Kurroo – a temperate medicinal herb. Jour. Pl. Sci. Res., 23(1-2): 69-72.
Kaur, R., Sood, M., Chander, S., Mahajan, R., Kumar, V. & Sharma, D.R. (1999). In vitro propagation of Valeriana jatamansi. Plant Cell Tissue and Organ Culture, 59(3): 227-229.
Niino, T., Sakai, A., Yakuwa, H. & Nojiri, K. (1992). Cryopreservation of in vitro grown shoot tips of apple and pear by vitrification. Plant Cell Tissue and Organ Culture, 28: 261-266.
Ouma, J.P., Young, M.M. & Reichert, N.A. (2004). Rooting of in vitro regenerated cotton. African Journal of Biotechnology, 13(6): 313-318.
Sakai, A., Kobayashi, S. & Olyama, I. (1990). Cryopreservation of nucellar cells of navel orange (Citrus sinensis) by vitrification. Plant Cell Reports, 9: 30-33.
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