In vitro Propagation and Conservation of Inula racemosa Hook. F. an Endangered Medicinal Plant of Temperate Origin

Authors

  • R. Kaur Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India.
  • Aditi Kashyap Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India.
  • Sadiq Majeed Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India.
  • N. S. Chauhan Department of Forest Products, Dr Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) 173230, India.
  • S. B. Bhardwaj Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India.

Keywords:

Vitrification, Inula racemosa, Micropropagation

Abstract

Inula racemosa is an endangered medicinal plant. It is commonly known as Pushkarmool, Pushkar and Manu. The great sage Charaka has characterized it as Hikka magrahana (stops hiccups) and Savasahara (helpful in asthma). Also, he has cited it as the best medicament for pleurisy along with cough and asthma. Due to the fragile nature of its habitat and exploitation due to its commercial medicinal properties, the species are facing the onslaught of indiscriminate over-exploitation. So far, this plant has not got the required attention from researchers, hence, except for a few efforts, not much work has been done for its cultivation and conservation. Plant tissue culture offers an attractive and quick method for its multiplication and further conservation. In the present investigation, effective procedures for micropropagation and in vitro conservation by vitrification were developed. In vitro propagation using aseptically grown seedlings and in vitro conservation via vitrification were standardized. The in vitro conserved material could be retrieved and multiplied normally on Murashige and Skoog medium fortified with 1.00 mg l-1BA (benzyl adenine) which has been recorded as the best performing medium for in vitro shoot multiplication. The conserved shoots showed normal in vitro propagation and after retrieval from vitrification, platelets were hardened and successfully established in the experimental fields under Nauni (Solan, HP) conditions at an elevation of around 1275 meters above mean sea level.

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References

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Published

01-07-2010

How to Cite

Kaur, R., Kashyap, A., Majeed, S., Chauhan, N. S., & Bhardwaj, S. B. . (2010). In vitro Propagation and Conservation of Inula racemosa Hook. F. an Endangered Medicinal Plant of Temperate Origin. Journal of Advanced Laboratory Research in Biology, 1(1), 67–70. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/20

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