Biological Properties of Actinomycetes Isolated from Marine Sponges in Madagascar

  • Rado Rasolomampianina Laboratoire de Microbiologie de l’Environnement, CNRE, Po Box 1739, Fiadanana, 101 Antananarivo, Madagascar.
  • Onja Andriambeloson Laboratoire de Microbiologie de l’Environnement, CNRE, Po Box 1739, Fiadanana, 101 Antananarivo, Madagascar.
  • Tokiniaina Rakotovao Laboratoire de Biotechnologie-Microbiologie, Département de Biochimie Fondamentale et Appliquée, Faculté des Sciences, PoBox 906, Ambohitsaina, 101 Université d’Antananarivo, Madagascar.
Keywords: Actinomycetes, Antimicrobial activity, Antioxidant activity, Marine sponges

Abstract

Marine actinomycetes are well known as a potential provider of novel bioactive compounds and currently considered as an important source of natural substances with unique chemical diversity. In this study, 20 marine actinomycetes were isolated from three Demospongia collected in the South East coast of Madagascar. Cultural, morphological, physiological and biochemical characteristics of the isolates showed that they belong to the genus Streptomyces. The Antimicrobial activity of the strains was performed using the agar cylinder technique against pathogens bacteria, yeast and fungi. It resulted that 90% of the isolates showed activity against at least one or more of the test germs. The isolates were more active against Gram-positive bacteria than Gram-negative bacteria. Simultaneously, ethanol extracts of the isolates were tested for their antioxidant activity using DPPH (1,1-Diphenyl-2-picrylhydrazyl) free radical scavenging test. Among tested extracts, those of Streptomyces M9 and M17 showed antioxidant activity against DPPH free radical with IC50 values of 12.8µg/ml and 12.4µg/ml, respectively.

Downloads

Download data is not yet available.

References

[1]. Mincer, T.J., Jensen, P.R., Kauffman, C.A. and Fenical, W. (2002). Widespread and persistent population of a major new marine actinomycete taxon in ocean sediments. Appl. Environ. Microbiol., 68:5005-5011.
[2]. Thangapandian, V., Ponmurugan, P. and Ponmurugan, K. (2007). Actinomycetes diversity in the rhizosphere soils of different medicinal plants in Kolly Hills-Tamilnadu, India, for secondary metabolite production. Asian J. Plant Sci., 6:66 -70.
[3]. Decre, D. and Courvalin P. (1995). De l'intérêt d'antibiotiques nouveaux. Bull. Soc. Fr. Microbiol., 12(2):160-164.
[4]. Li, J.W.H. and Vederas, J.C. (2009). Drug discovery and natural products: end of an era or an endless frontier? Science, 325:161-165.
[5]. Bull, A.T., Stach, J.E.M. (2007). Marine actinobacteria: new opportunities for natural product search and discovery. Trends Microbiol., 15:491-499.
[6]. Buchanan, G.O., Williams, P.G., Feling, R.H., Kauffman, C.A., Jensen, P.R. and Fenical, W. (2005). Sporolides A and B: structurally unprecedented halogenated macrolides from the marine actinomycete Salinispora tropica. Org. Lett., 7(13):2731-2734.
[7]. Fiedler, H.P., Bruntner, C., Bull, A.T., Ward, A.C., Goodfellow, M., Potterat, O., Puder, C., Mihm, G. (2005). Marine actinomycetes as a source of novel secondary metabolites. Antonie Van Leeuwenhoek, 87:37-42.
[8]. Jensen, P.R., Mincer, T.J., Williams, P.G. and Fenical, W. (2005). Marine actinomycete diversity and natural product discovery. Antonie Van Leeuwenhoek., 87:43–48.
[9]. Jensen, P.R., Williams, P.G., Oh, D.C., Zeigler, L. and Fenical, W. (2007). Species-specific secondary metabolite production in marine actinomycetes of the genus Salinispora. Appl. Environ. Microbiol., 73:1146-1152.
[10]. Izumikawa, M., Khan, S.T., Komaki, H., Takagi, M. and Shin-Ya, K. (2010a). JBIR-31, a new teleocidin analog, produced by a salt-requiring Streptomyces sp. NBRC 105896 isolated from a marine sponge. J. Antibiot., 63:33-36.
[11]. Khan, S.T., Izumikawa, M., Motohashi, K., Mukai, A., Takagi, M. and Shin-Ya, K. (2010a). Distribution of the 3-hydroxyl-3-methylglutaryl coenzyme A reductase gene and isoprenoid production in marine-derived Actinobacteria. FEMS Microbiol. Lett., 304:89-96.
[12]. Izumikawa, M., Khan, S.T., Takagi, M. and Shin-Ya, K. (2010b). Sponge-derived Streptomyces producing isoprenoids via the mevalonate pathway. J. Nat. Prod., 73:208-212.
[13]. Acar, J.F. and Goldstein, F.W. (1996). Disk Susceptibility Test. In: V. Lorian. “Antibiotics in Laboratory Medicine”. Baltimore: Williams and Wilkins Co., 4th Ed., 1-51.
[14]. Kekuda, P.T.R., Shobha, K.S. and Onkarappa, R. (2010). Studies on antioxidant and anthelmintic activity of two Streptomyces species isolated from Western Ghat soil of Agumbe, Karnataka. Journal of Pharmacy Research, 3(1), 26-29.
[15]. Basilio, A., González, I., Vicente, M.F., Gorrochategui, J., Cabello, A., González, A., Genilloud, O. (2003). Patterns of antimicrobial activities from soil actinomycetes isolated under different conditions of pH and salinity. J. Appl. Microbiol., 95:814-823.
[16]. Oskay, M., Tamer, A.Ü. and Azeri, C. (2004). Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. Afr. J. Biotechnol., 3:441-446.
[17]. Sacramento, D.R., Coelho, R.R.R., Wigg, M.D., Linhares, L.F.T.L., Santos, M.G.M., Semêdo, L.T.A.S. and Silva, A.J.R. (2004). Antimicrobial and antiviral activities of an actinomycete (Streptomyces sp.) isolated from a Brazilian tropical forest soil. World J. Microbiol. Biotechnol., 20: 225-229.
[18]. Thenmozhi, M. and Kannabiran, K. (2012). Antimicrobial and antioxidant properties of marine actinomycetes Streptomyces sp. VITSTK7. Oxid. Antioxid. Med. Sci., 1(1):51-57.
[19]. Thenmozhi, M., Sindhura, S. and Kannabiran, K. (2010). Characterization of Antioxidant activity of Streptomyces species VITTK3 isolated from Puducherry Coast, India. Journal of Advanced Scientific Research, 1(2): 46-52.
[20]. Janardhan, A., Kumar, A.P., Viswanath, B., Saigopal, D.V.R. and Golla Narasimha (2014). Production of Bioactive Compounds by Actinomycetes and Their Antioxidant Properties. Biotechnology Research International, vol. 2014, Article ID 217030, 8 pages, 2014. https://doi.org/10.1155/2014/217030.
Published
2015-01-01
How to Cite
Rasolomampianina, R., Andriambeloson, O., & Rakotovao, T. (2015). Biological Properties of Actinomycetes Isolated from Marine Sponges in Madagascar. Journal of Advanced Laboratory Research in Biology, 6(1), 1-5. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/215
Section
Articles
Abstract viewed = 53 times, PDF downloaded = 32 times