Mutagenic Effects of Sodium Azide on the Quality of Maize Seeds

  • J. J. Eze Department of Biological Sciences, Ahmadu Bello University, Zaria, Nigeria.
  • A. Dambo Department of Biological Sciences, Ahmadu Bello University, Zaria, Nigeria.
Keywords: Mutation, Mutagen, Mutagenic, Sodium Azide, Mutants, Maize, Varieties, Nutrients

Abstract

This project was conducted to determine the mutagenic effects of sodium azide on the quality of maize seed. Maize seeds were treated at six different concentrations of sodium azide namely; 0.00mM, 0.01mM, 0.02mM, 0.03mM, 0.04mM and 0.05mM. The seeds that were treated were of two varieties namely; Sammaz 18 and Sammaz 20. The result obtained showed that sodium azide was effective in causing mutagenic change in the quality of maize seeds in terms of growth rate and seed size. Significant differences (P<0.05) were observed in all the two varieties with respect to some of the traits studied and nutritional compositions studied. The number of days to 50% flowering and Nitrogen-free extract (%) increased significantly with an increase in concentrations of sodium azide. Chlorophyll-deficient mutants were observed in treatments 0.02mM, 0.03mM and 0.04mM which were striata and light green in colour. Dwarfed mutant was also recorded in treatment 0.04mM of Sammaz 20 maize variety. The project was carried out to find out the effectiveness of sodium azide on the mutagenesis of maize seeds, seed weight of mutant maize plant, nutrient content of the mutant maize plant and the morphological features of the mutant maize plant. I recommend that chemical mutagens like sodium azide to produce improved seed varieties like of maize plants that will meet the present global and national food need.

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References

[1]. Acharya, S.N., Thomas, J.E. and Basu, S.K, (2007). Improvement in the medicinal and nutritional properties of fenugreek (Trigonella foenum-graecum L.). In: S.N. Acharya, J.E. Thomas (Eds.) Advances in Medicinal Plant Research, Research Signpost, Trivandrum, Kerala, India.
[2]. Adamu, A.K. and Aliyu, H. (2007). Morphological effects of sodium azide on tomato (Lycopersicon esculentum Mill). Science World Journal, 2(4): 9-12.
[3]. Adamu, A.K. (2004). Gamma rays (60Co) and thermal neutron induced mutants in popcorn (Zea mays var parecox sturt): Nigerian Journal of Scientific Research, 4(2):52-63.
[4]. Adamu, A.K., Olorunju, P.E., Bate, J.A. and Ogunlade, O.T.E. (2002). Radiosensitivity and effective dose determination in groundnut (Arachis hypogea L.) Irradiation with gamma rays (Cobalt 60) sources. Journal of Agriculture and Environment, 3(1): 71-84.
[5]. Ahloowalia, B.S. and Maluszynski, M. (2001). Induced mutation-A new paradigm in plant Breeding. Euphytica, 118:167-173.
[6]. Bretagne-Sagnard, B., Fouilloux, G., Chupeau., Y. (1996). Induced Albina mutations as a tool for genetic analysis and cell biology in flax (Linum usitatissimum). Journal of Experimental Botany, 47:189-194.
[7]. Deepalakshmi, A.J. and Anandakumar, C.R. (2004). Creation of genetic variability for different polygenic traits in black gram (Vigna mungo (L.) Hepper) through induced mutagenesis. Legume Research, 27(3): 188-192.
[8]. Dubinin, N.P. (1961). Problems of radiation genetics. Oliver & Boyd, London.
[9]. Osorio, J., Fernández-Martínez, J., Mancha, M. and Garcés, R. (1995). Mutant sunflowers with high concentration of saturated fatty acids in the oil. Crop Science, 35: 739-742.
[10]. Gichner, T. and Velemínský, J. (1977). The very low mutagenic activity of sodium azide in Arabidopsis thaliana. Biologia Plantarum, 19(2):153–155.
[11]. Girija, M. (2008). Studies on induced mutagenesis in cowpea (Vigna unguiculata (L.) Walp). M. Phil. Thesis. Annamalai University, Annamalai Nagar, Tamil Nadu.
[12]. Gnanamurthy, S., Dhanavel, D., Girija, M., Pavadai, P. and Bharathi, T. (2012). Effect of chemical mutagenesis on quantitative traits of Maize (Zea mays L.). International Journal of Research in Botany, 2(4): 34-36.
[13]. Hagberg, A. (1962). Production of duplications in barley breeding. Hereditas, 48: 243-246.
[14]. Jones, J.A., Starkey, J.R. and Kleinhofs, A. (1980). Toxicity and mutagenicity of sodium azide in mammalian cultures. Mutation Research, 77: 293-299.
[15]. Ramani, G.M. and Jadon, B.S. (1991). Induced variability in groundnut in M2 generation. Gujarat Agricultural University Research Journal, 16(2):23-26.
[16]. Kamra, O.P. and Gollapudi, B. (1979). Mutagenic effects of sodium azide in Drosophila melanogaster. Mutation Research, 66: 381-384.
[17]. Lee, Y.I., Lee, I.S. and Lim, Y.P. (2002). Variations in sweet potato regenerates from gamma-ray irradiated embryogenic callus. Journal of Plant Biotechnology, 4:163-170.
[18]. Maluszynski, M., Nichterlein, K., Zanten, L. van, & Ahloowalia, B.S. (2000). Officially released mutant varieties - the FAO/IAEA Database (INIS-XA--291). International Atomic Energy Agency (IAEA): IAEA.
[19]. Gramatikova, M. and Todorov, Y. (1996). Mutagenic specificity recording micro and macro mutants induced. Genetic Newsletter, 27:68-72.
[20]. Owais, W.M. and Sadiq, M.F. (1993). O-acetyl-serine sulfhydrylases from different organisms converts azide to a mutagenic metabolite: Ability of the metabolite to inhibit the enzyme in vitro. Abhath Al-Yarmouk, 2(2): 43-55.
[21]. Pavadai, P. and Dhanavel, D. (2004). Effect of EMS, DES and colchicine treatment in soybean. Crop Research, 28: 118-120.
[22]. Pugalendi, N. (1992). Investigation on induced mutagenesis in Sesamum indicum L. M.Sc. (Ag.) Thesis, Annamalai University, Annamalai Nagar, Tamil Nadu.
[23]. Ramaswamy, N.M. (1973). Investigation on induced mutagenesis in black gram (Phaseolus mungo L.). Ph.D. Thesis, Tamil Nadu Agri. Univ., Coimbatore.
[24]. Rao, G.M. and Rao, V.M. (1983). Mutagenic efficiency, effectiveness and factor of effectiveness of physical and chemical mutagens in rice. Cytologia, 48:427-436.
[25]. Ricardo, M. and Ando, A. (1980). Effect of gamma-radiation and sodium azide on quantitative characters in rice (Oryza sativa L.). Genetics and Molecular Biology, 21:11-19.
[26]. Rines, H.W. (1985). Sodium azide mutagenesis in diploid and hexaploid oats and comparison with ethyl methanesulfonate treatments. Environmental and Experimental Botany, 25:7-16.
[27]. Rizwana, B.M., Kalamani, A., Ashok, S., Makesh, S. (2005). Effect of mutagenic treatments on qualitative characters in M1 generation of cowpea [Vigna unguiculata (L.) Walp]. Adv. Plant Sci., 18(2): 505-510.
[28]. Sasi, A., Dhanavel, D. and Pavadai, P. (2005). Effect of chemical mutagenesis on bhendi [Abelmoschus esculentus (L.) Moench] var. MDU-1. Research on Crops, 6(2): 300-306.
[29]. Singh, S.D., King, S.B. and Reddy, P.M. (1990) Registration of five pearl millet germplasm sources with stable resistance to downy mildew. Crop Science, 30(5): 1164.
[30]. Tarar, J.L. and Dnyansagar, V.R. (1980). Comparison of ethyl methane sulphonate and radiation induced meiotic abnormalities in Turnera ulmifolia Linn. Var. angustifolia Wild. Cytologia, 45:221–231.
[31]. van den Bulk, R.W., Löffler, H.J., Lindhout, W.H. and Koornneef, M. (1990). Somaclonal variation in tomato: effect of explant source and a comparison with chemical mutagenesis. Theoretical and Applied Genetics, 80:817-825.
[32]. Wen, J.G. and Liang, H.G. (1995). Effect of KCN and NaN3 pretreatment on the cyanide resistant respiration in tobacco callus. Acta Bot. Sin., 37:711-717.
[33]. Yuan, H.Y and Zhang, Z. (1993). Effect of Free Radicals and Temperature on Sister Chromatid Exchanges in Hordeum vulgare L. Acta Botanica Sinica, 35: 20-26.
Published
2015-07-01
How to Cite
Eze, J., & Dambo, A. (2015). Mutagenic Effects of Sodium Azide on the Quality of Maize Seeds. Journal of Advanced Laboratory Research in Biology, 6(3), 76-82. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/230
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Articles
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