Isolation and Identification of Pathogenic Bacteria that Cause External Ocular Infections in Sabha City Libya


  • Abdelkader Alsanousi G. Elzen Faculty of Science, Sabha University, P.O. Box 18758, Sabha-Libya.
  • Alsadig Mohammed Abdalla Department of Microbiology, Faculty of Medicine, Sabha University, P.O. Box 18758, Sabha-Libya.


Ocular Infections, Isolation, Identification of pathogenic bacteria


The aim of this study was the isolation and identification of the specific bacterial pathogens causing ocular infections in patients attending Sabha Eye Clinics. A total of 120 samples were collected using a sterile cotton swab from patients with conjunctivitis (aged between 1 to more than 60 years) attending different Eye Clinics in Sabha city (under the supervision of ophthalmologists). All samples were examined by Gram stain, culture on blood agar, mannitol salt agar and MacConkey agar. Different biochemical tests were studied. Results revealed that the species of bacteria isolated were: Staphylococcus aureus (40%), Coagulase-negative Staphylococci (20%), Klebsiella pneumoniae species (10%), Pseudomonas aeruginosa (22%) and E. coli 8%.


Download data is not yet available.


[1]. Alvarez, H., Tabbara, K.F. (1996). Infections of the eyelid. In: Tabbara K.F., Hyndiuk R.A., editors. Infections of the Eye. 2nd ed. Boston: Little Brown and Company, pp. 559-570.
[2]. Barrow, G.I. and Feltham, R.K.A. (2003). Cowan and Steel’s Manual for Identification of the Medical Bacteria. 3rd edition. Cambridge University Press, Cambridge, U.K. pp. 45-120.
[3]. Bauman, Robert W. (2012). Microbiology: with diseases by body system; Contributions by Elizabeth Machunis-Masuoka, Jean E. Montgomery; Clinical consultants, Cecily D. Cosby, Janet Fulks, John M. Lammert. – 3rd ed. Copyright© 2012, Pearson Education, Inc., publishing as Benjamin Cummings.
[4]. Briuser, J.H. (1996). Ocular Bacteriology. Infections of the Eye. 2nd Edn. Little Brown and Company, Boston, pp 137.
[5]. Esenwah, E. (2005). Isolation and identification of the microorganisms most prevalent in external eye infections as seen in an eye clinic in Owerri. Journal of the Nigerian Optometric Association, 12:6-9.
[6]. Hemavathi, Sarmah, P., Shenoy, P. (2014). Profile of Microbial Isolates in Ophthalmic Infections and Antibiotic Susceptibility of the Bacterial Isolates: a study in an eye care hospital, Bangalore. Journal of Clinical and Diagnostic Research, 8(1): 23-25.
[7]. McClellan, K.A. (1997). Mucosal defense of the outer eye. Surv. Ophthalmol., 42:233-46.
[8]. Ogbolu, D.O., Alli, O.A.T., Ephraim, I.E., Olabiyi, F.A., Daini, O.A. (2011). In-vitro efficacy of Antimicrobial Agents used in the Treatment of Bacterial Eye Infections in Ibadan, Nigeria. African Journal of Clinical and Experimental Microbiology, 12(3): 124-128. DOI: 10.4314/ajcem.v12i3.7
[9]. Kenneth J. Ryan and C. George Ray (2004). Sherris Medical Microbiology: An Introduction to Infectious Diseases, 4th Edition, McGraw-Hill Medical Publishing Division, by the McGraw-Hill Companies.
[10]. Sherwal, B.L. and Verma, A.K. (2008). Epidemiology of Ocular Infection Due to Bacteria and Fungus – A Prospective Study. Journal of Medical Education & Research, 10(3): 127-131.
[11]. Sharma, S. (1988). Ocular Microbiology. 1st ed. Arvind Eye Hospital Publication, Madurai.
[12]. Stenson, S., Newman, R., Fedukowicz, H. (1982). Laboratory studies in acute conjunctivitis. Arch. Ophthalmol., 100:1275-7.
[13]. Tesfaye, T., Beyene, G., Gelaw, Y., Bekele, S., Saravanan, M. (2013). Bacterial Profile and Antimicrobial Susceptibility Pattern of External Ocular Infections in Jimma University Specialized Hospital, Southwest Ethiopia. American Journal of Infectious Diseases and Microbiology, 1(1): 13-20. DOI: 10.12691/ajidm-1-1-3.




How to Cite

Elzen, A. A. G., & Abdalla, A. M. (2015). Isolation and Identification of Pathogenic Bacteria that Cause External Ocular Infections in Sabha City Libya. Journal of Advanced Laboratory Research in Biology, 6(4), 95–96. Retrieved from




Most read articles by the same author(s)