Prevalence and Antibiotic Susceptibility Pattern of Pseudomonas aeruginosa Isolated from Hospital Environment in South Libya


  • Ibrahim Ali Altayyar Department of Medical Laboratory Sciences, Faculty of Engineering and Technology, Sebha University, Sebha-Libya.
  • Alsadig Mohammed Abdalla Department of Microbiology, Faculty of Medicine, Sebha University, Sebha-Libya.
  • Abdelkader Alsanousi G. Elzen Department of Microbiology, Faculty of Science, Sebha University, Sebha-Libya.
  • Mohamed Farg Elbreki Department of Medical Laboratory Sciences, Faculty of Engineering and Technology, Sebha University, Sebha-Libya.


Pseudomonas aeruginosa, Hospital contamination, Antibiotic susceptibility pattern


Pseudomonas aeruginosa has been emerged as a significant pathogen and is the most common dreadful gram-negative bacilli found in various health care-associated infections all over the world due to its virulence, well-known ability to resist killing by various antibiotics and disinfectants. The aim of this study was isolation and identification of Pseudomonas aeruginosa in the hospital environment and determining the antibiotic susceptibility of the isolates to four antibiotics (Ciprofloxacin, Amikacin, Imipenem, and Piperacillin).

A total of 200 sterile cotton swab samples were collected from hospital environment including ground, walls, beds, bed sheets, blankets, doors, doors handle, nurse tables, trays, chairs, electronic equipment's, medicine cabinet, windows and (operation theater) (Sabha medical center and Brack general hospital were enrolled in this cross-sectional study). Bacterial isolates were identified by standard microbiological procedures. Antibiotic susceptibility testing was carried out by disc diffusion method.

Results revealed that out of the 200 collected samples, 12 Pseudomonas spp. (6%) were isolated. Other different bacterial species isolated were 148 (74%) and 40 samples (20%) were negative for growth. Most isolates were obtained from sinks 6 (50%) and then ground 2 (16.7%), Air conditions 2 (16.7%), walls 1 (8.3%), Chairs 1 (8.3%). we found that all Pseudomonas spp. isolates were sensitive to Ciprofloxacin, Amikacin, Piperacillin, and Imipenem.


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How to Cite

Altayyar, I. A., Abdalla, A. M., Elzen, A. A. G., & Elbreki, M. F. (2016). Prevalence and Antibiotic Susceptibility Pattern of Pseudomonas aeruginosa Isolated from Hospital Environment in South Libya. Journal of Advanced Laboratory Research in Biology, 7(2), 43–46. Retrieved from