Prevalence and Antibiotic Susceptibility Pattern of Pseudomonas aeruginosa Isolated from Hospital Environment in South Libya
Keywords:Pseudomonas aeruginosa, Hospital contamination, Antibiotic susceptibility pattern
Pseudomonas aeruginosa has been emerged as a significant pathogen and is the most common dreadful gram-negative bacilli found in various health care-associated infections all over the world due to its virulence, well-known ability to resist killing by various antibiotics and disinfectants. The aim of this study was isolation and identification of Pseudomonas aeruginosa in the hospital environment and determining the antibiotic susceptibility of the isolates to four antibiotics (Ciprofloxacin, Amikacin, Imipenem, and Piperacillin).
A total of 200 sterile cotton swab samples were collected from hospital environment including ground, walls, beds, bed sheets, blankets, doors, doors handle, nurse tables, trays, chairs, electronic equipment's, medicine cabinet, windows and (operation theater) (Sabha medical center and Brack general hospital were enrolled in this cross-sectional study). Bacterial isolates were identified by standard microbiological procedures. Antibiotic susceptibility testing was carried out by disc diffusion method.
Results revealed that out of the 200 collected samples, 12 Pseudomonas spp. (6%) were isolated. Other different bacterial species isolated were 148 (74%) and 40 samples (20%) were negative for growth. Most isolates were obtained from sinks 6 (50%) and then ground 2 (16.7%), Air conditions 2 (16.7%), walls 1 (8.3%), Chairs 1 (8.3%). we found that all Pseudomonas spp. isolates were sensitive to Ciprofloxacin, Amikacin, Piperacillin, and Imipenem.
Andrade, S.S., Jones, R.N., Gales, A.C. & Sader, H.S. (2003). Increasing prevalence of antimicrobial resistance among Pseudomonas aeruginosa isolates in Latin American medical centres: 5 year report of the SENTRY Antimicrobial Surveillance Program (1997-2001). J. Antimicrob. Chemother., 52(1): 140–141. https://doi.org/10.1093/jac/dkg270.
Boyce, J.M. (2007). Environmental contamination makes an important contribution to hospital infection. J. Hosp. Infect., 65(Suppl 2): 50–54. https://doi.org/10.1016/S0195-6701(07)60015-2.
Boyce, J.M. & Pittet, D. (2002). Guideline for Hand Hygiene in Health-Care Settings: Recommendations of the Healthcare Infection Control Practices Advisory Committee and the HICPAC/SHEA/APIC/IDSA Hand Hygiene Task Force. Infect. Control Hosp. Epidemiol., 23(S12): S3–S40. https://doi.org/10.1086/503164.
Carling, P.C., Parry, M.F. & Von Beheren, S.M. (2008). Identifying opportunities to enhance environmental cleaning in 23 acute care hospitals. Infect. Control Hosp. Epidemiol., 29(1): 1–7. https://doi.org/10.1086/524329.
Filetoth, Z. (2003). Hospital-Acquired Infection: Causes and Control. Whurr Publishers Ltd., UK.
Hayden, M.K., Bonten, M.J., Blom, D.W., Lyle, E.A., van de Vijver, D.A. & Weinstein, R.A. (2006). Reduction in acquisition of vancomycin-resistant enterococcus after enforcement of routine environmental cleaning measures. Clin. Infect. Dis., 42(11): 1552–1560. https://doi.org/10.1086/503845.
Kramer, A., Schwebke, I. & Kampf, G. (2006). How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infect. Dis., 6: 130. https://doi.org/10.1186/1471-2334-6-130.
Savaş, L., Duran, N., Savaş, N., Önlen, Y. & Ocak, S. (2005). The Prevalence and Resistance Patterns of Pseudomonas aeruginosa in Intensive Care Units in a University Hospital. Turkish Journal of Medical Sciences, 35(5): 317–322.
Ghane, M. & Azimi, Z. (2014). Isolation, Identification and Antimicrobial Susceptibility of Pseudomonas spp. Isolated from Hospital Environment in Tonekabon, North of Iran. J. Appl. Environ. Microbiol., 2(4): 97–101.
Nasreen, M., Sarker, A., Malek, M.A., Ansaruzzaman, Md. & Rahman, M. (2015). Prevalence and Resistance Pattern of Pseudomonas aeruginosa Isolated from Surface Water. Advances in Microbiology, 5(1): 74–81. http://dx.doi.org/10.4236/aim.2015.51008.
Nseir, S., Blazejewski, C., Lubret, R., Wallet, F., Courcol, R. & Durocher, A. (2011). Risk of acquiring multidrug-resistant Gram-negative bacilli from prior room occupants in the intensive care unit. Clin. Microbiol. Infect., 17(8): 1201–1208. https://doi.org/10.1111/j.1469-0691.2010.03420.x.
Pal, R.B., Rodrigues, M. & Datta, S. (2010). Role of Pseudomonas in Nosocomial Infections and Biological Characterization of Local Strains. J. Biosci. Tech., 1(4): 170-179.
Panagea, S., Winstanley, C., Walshaw, M.J., Ledson, M.J. & Hart, C.A. (2005). Environmental contamination with an epidemic strain of Pseudomonas aeruginosa in a Liverpool cystic fibrosis centre, and study of its survival on dry surfaces. J. Hosp. Infect., 59(2): 102–107. https://doi.org/10.1016/j.jhin.2004.09.018.
Penna, V.T., Martins, S.A. & Mazzola, P.G. (2002). Identification of bacteria in drinking and purified water during the monitoring of a typical water purification system. BMC Public Health, 2: 13. https://doi.org/10.1186/1471-2458-2-13.
Pirnay, J.P., Matthijs, S., Colak, H., Chablain, P., Bilocq, F., Van Eldere, J., De Vos, D., Zizi, M., Triest, L. & Cornelis, P. (2005). Global Pseudomonas aeruginosa biodiversity as reflected in a Belgian river. Environ. Microbiol., 7(7): 969–980. https://doi.org/10.1111/j.1462-2920.2005.00776.x.
Prakash, H.R., Belodu, R., Karangate, N., Sonth, S., Anitha, M.R. & Vijayanath, V. (2012). Antimicrobial susceptibility pattern of Pseudomonas aeruginosa strains isolated from clinical sources. J. Pharm. Biomed. Sci., 14: 1-4.
Senthamarai, S., Reddy, A.S., Sivasankari, S., Anitha, C., Somasunder, V., Kumudhavathi, M.S., Amshavathani, S.K. & Venugopal, V. (2014). Resistance Pattern of Pseudomonas aeruginosa in a Tertiary Care Hospital of Kanchipuram, Tamilnadu, India. J. Clin. Diagn. Res., 8(5): DC30–DC32. https://doi.org/10.7860/JCDR/2014/7953.4388.
Uma Maheswaran, S.K., Meenakshi Sundaram, M. & Rajasekaran, S. (2007). A Study on Controlling Hospital Acquired Infections: A Knowledge Based System Approach. Information Technology Journal, 6: 129-134. https://dx.doi.org/10.3923/itj.2007.129.134.
WHO (2002). Prevention of hospital-acquired infections: a practical guide. Ducel, G., Fabry, J. & Nicolle, L. (eds.), 2nd ed., World Health Organization, Geneva. https://apps.who.int/iris/handle/10665/67350.
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