Antigenic Detection of Salmonella Infection among Pediatric Patients with Acute Gastroenteritis
Keywords:Acute diarrhea, Salmonella, Abdominal pain, Immunochromatography
Background: Diarrheal diseases are one of the social problems in developing countries. The pathogens commonly associated with childhood diarrhea are Salmonella, Clostridium difficile, Shigella, Yersinia and Escherichia coli but the highest attack rate for salmonellosis in infancy.
Objective: The aim of the study was to evaluate the presence of Salmonella antigen in acute gastroenteritis in children admitted to a pediatric hospital.
Material and Methods: The study was performed on freshly collected stool samples among 94 acute diarrheal children below two years admitted to AL-Khadymia and AL-Elweya pediatric hospitals from May 2015 to January 2016. A questionnaire was completed for each patient’s name, age, gender, clinical data like fever, nausea, vomiting, and abdominal pain. The criteria included hemorrhagic fresh stool sample in addition to containing parasite agent. Fresh stool samples were tested by immunochromatographic assay for antigenic detection of Salmonella.
Results: Salmonella antigen identified in five stool samples one for male and four for females. All pediatric patients show fever, vomiting and abdominal pain, while the stool consistency distributed to 75.5% watery and 24.5% loosely. Stool samples show 69.1% with blood and 39.9% without blood, 16.9% with pus and 83.1% without pus, 83% with mucous and 17% without mucous. Four cases with giardiasis and 24 cases with entamebiasis and 14 cases with cyst of E. histolytica or G. lamblia in addition to absence the parasites ova in all stool samples.
Conclusion: Salmonella antigen present in five stool samples, all the patients show vomiting, fever, abdominal pain, 65 cases with blood in comparison with, 29 without blood 15 cases with pus in comparison with 79 without pus. 78 cases with mucous in comparison with, 16 without mucous, four cases with goddesses and 24 cases with entamebiasis, 14 cases with cyst of E. histolytica or G. lamblia in addition to absence the parasites ova in all stool samples.
. Thielman, N.M. & Guerrant, R.L. (2004). Acute Infectious Diarrhea. N. Engl. J. Med., 350(1): 38-47.
. World health organization (2009). Diarrheal Diseases. Geneva: WHO.
. Anonymous (2000). Salmonella serotypes recorded in the Public Health Laboratory Service Salmonella data set, April to June 2000. Communicable Disease Report, 10: 332.
. Casalino, M., Yusuf, M.W., Nicoletti, M., Bazzicalupo, P., Coppo, A., Colonna, B., Cappelli, C., Bianchini, C., Falbo, V., Ahmed, H.J., Omar, K.H., Maxamuud, K.B. & Maimone, F. (1988). A two-year study of enteric infections associated with diarrhoeal diseases in children in urban Somalia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 82: 637–641.
. Cohen, M.B. & Laney, D.W. Jr., (1999). Infectious diarrhea. In: Wyllie, R., Hyams, J.S., eds. Pediatric Gastrointestinal Disease: Pathophysiology, Diagnosis and Management. 2nd edn. Philadelphia: WB Saunders. ISBN 0-7216-7461-5. pp. 348–370.
. World Health Organization (1983). World health: the magazine of the World Health Organization. 10: 14-16.
. Cobbold, R.N., Rice, D.H., Davis, M.A., Besser, T.E. & Hancock, D.D. (2006). Long-term persistence of multi-drug-resistant Salmonella enterica serovar Newport in two dairy herds. J. Am. Vet. Med. Assoc., 228(4): 585-591.
. Akinterinwa, M.O. & Paul, M.O. (1982). Bacteriological investigations of infantile gastroenteritis in Ife, Nigeria. J. Trop. Med. Hyg., 85(4): 139-141.
. Guerrant, R.L., Shields, D.S., Thorson, S.M., Schorling, J.B. & Gröschel, D.H. (1985). Evaluation and diagnosis of acute infectious diarrhea. Am. J. Med., 78(6B): 91-98.
. Shobirin, M.A., Shuhaimi, M., Abu-Bakar, F., Ali, A.M., Ariff, A., Nur-Atiqah, N.A. & Yazid, A.M. (2003). Characterization of Salmonella spp. isolated from patients below 3 years old with acute diarrhoea. World Journal of Microbiology and Biotechnology, 19: 751-755.
. Nelson, J.D. (1985). Etiology and epidemiology of diarrheal diseases in the United States. Am. J. Med., 78(6B): 76-80.
. Sanyal, S.C., Sen, P.C., Tiwari, I.C., Bhatia, B.D. & Singh, S.J. (1977). Microbial agents in stools of infants and young children with and without acute diarrhoeal disease. J. Trop. Med. Hyg., 8(1): 2-8.
. Taylor, D.N., Echeverria, P., Pal, T., Sethabutr, O., Saiborisuth, S., Sricharmorn, S., Rowe, B. and Cross, J. (1986). The role of Shigella spp., enteroinvasive Escherichia coli, and other enteropathogens as causes of childhood dysentery in Thailand. J. Infect. Dis., 153(6): 1132-1138.
. Smith, J.L. & Bayles, D. (2007). Postinfectious irritable bowel syndrome: a long-term consequence of bacterial gastroenteritis. J. Food Prot., 70(7): 1762-1769.
. Santos, R.L., Zhang, S., Tsolis, R.M., Kingsley, R.A., Adams, L.G. & Bäumler, A.J. (2001). Animal models of Salmonella infections: enteritis versus typhoid fever. Microbes Infect., 3(14-15): 1335-1344.
. Esch, K.J., & Petersen, C.A. (2013) Transmission and epidemiology of zoonotic protozoal diseases of companion animals. Clin. Microbiol. Rev., 26(1): 58-85. doi: 10.1128/CMR.00067-12.
. Bazzaz, A.A. & Ahmad, N.A. (2016). Prevalence of some parasitic infectious diseases within Kerkuk City for years 2009-2014. EJMPR, 3(6): 13-16.
. Stark, D., van Hal, S., Marriott, D., Ellis, J. & Harkness, J. (2007). Irritable bowel syndrome: a review on the role of intestinal protozoa and the importance of their detection and diagnosis. Int. J. Parasitol., 37(1): 11-20.
. Farrar, J., Hotez, P., Junghanss, T., Kang, G., Lalloo, D. & White, N. (2013). Manson's Tropical Diseases. 23rd Edition, Elsevier Health Sciences, 664–671. ISBN 9780702053061.
. Haque, R., Mondal, D., Duggal, P., Kabir, M., Roy, S., Farr, B.M., Sack, R.B. & Petri, W.A. Jr., (2006). Entamoeba histolytica infection in children and protection from subsequent amebiasis. Infect. Immun., 74(2): 904-909.