Trichoderma asperellum, a potential fungal biocontrol agent against Aspergillus niger

  • Rupa Verma Department of Botany, Ranchi University, Ranchi 834001, Jharkhand, India. https://orcid.org/0000-0001-9363-0623
  • Abhijit Dutta Department of Zoology, Ranchi University, Ranchi 834001, Jharkhand, India.
  • Ashok Kumar Choudhary Department of Botany, Ranchi University, Ranchi 834001, Jharkhand, India.
  • Sudarshan Maurya ICAR–Research Complex for Eastern Region, Research Centre, Plandu, Ranchi 834010, Jharkhand, India.
Keywords: Trichoderma asperellum, Aspergillus niger, Black mould, Antagonistic potential, Inhibition

Abstract

Trichoderma asperellum is free-living, ubiquitous fungus which is very common in the habitat of soil and root ecosystem, is known as a potent opportunistic, avirulent plant symbionts and it parasitizes several soilborne phytopathogens. Aspergillus niger is well known black mold which causes several storage diseases. Among the storage diseases, black mould disease of onion is an important disease which is caused by A. niger. Antagonsitic potential of T. asperellum was assayed against three isolates of A. niger [RC1, RC2 (isolated from soil samples of Farm 1 and 2) and RC3 isolated from diseases onion]. Antagonistic efficacy of T. asperellum of A. niger almost similar against all the test isolates. Percentage inhibition of radial growth (PIRG) of A. niger by T. asperellum inhibited 55.17% within five days, 77.20% within 7 days and 92.06% in 12 days. Antagonistic efficacy of T. asperellum can be exploited in the management of black mould disease of onion.

Downloads

Download data is not yet available.

References

[1]. Rai, N. & Yadav, D.S. (2005). Onion. Chapter 2.2. Advances in Vegetable Production. Researchco Book Centre, New Delhi.
[2]. Özer, N. & Köycü, N.D. (2004). Seed-borne Fungal Diseases of Onion, and their control. In: Mukerji K.G. (eds) Fruit and Vegetable Diseases. Disease Management of Fruits and Vegetables, vol 1. Springer, Dordrecht. pp 281-306.
[3]. Srinivasan, R. & Shanmugam, V. (2006). Post harvest management of black mould rot of onion. Indian Phytopathology, 59(3): 333-339.
[4]. Hayden, N.J. & Maude, R.B. (1992). The role of seed‐borne Aspergillus niger in transmission of black mould of onion. Plant Pathology, 41: 573-581. doi:10.1111/j.1365-3059.1992.tb02456.x.
[5]. Baker, R. & Paulitz, T.C. (1996). Theoretical basis for microbial interactions leading to biological control of soil borne plant pathogens In: Hall, R. (ed). Principles and Practice of Managing Soilborne Plant Pathogens. Am. Phythopathol. Soc. St. Paul, MN. pp 50-79.
[6]. Harman, G.E., Howell, C.R., Viterbo, A., Chet, I. & Lorito, M. (2004). Trichoderma species-opportunistic, avirulent plant symbionts. Nat. Rev. Microbiol., 2(1): 43-56.
[7]. Maurya, S., Singh, R., Singh, D.P., Singh, H.B., Singh, U.P. & Srivastava, J.S. (2008). Management of collar rot of chickpea (Cicer arietinum) by Trichoderma harzianum and plant growth promoting Rhizobacteria. Journal of Plant Protection Research, 48(3): 347–354. doi: 10.2478/v10045-008-0044-3.
[8]. Kumar, R., Maurya, S., Kumari, A., Choudhary, J., Das, B., Naik, S.K. and Kumar, S. (2012). Biocontrol Potentials of Trichoderma Harzianum against Sclerotial Fungi. The Bioscan, 7(3): 521-525.
[9]. Rashmi, S. Maurya, S. & Upadhyay, R.S. (2016). The improvement of competitive saprophytic capabilities of Trichoderma species through the use of chemical mutagens. Braz. J. Microbiol., 47(1): 10-17. doi: 10.1016/j.bjm.2015.11.003.
[10]. Maurya, S., Singh, R., Singh, D., Singh, H., Singh, U. & Srivastava, J. (2008). Management of Collar Rot of Chickpea (Cicer Arietinum) by Trichoderma Harzianum and Plant Growth Promoting Rhizobacteria. Journal of Plant Protection Research, 48(3): 347-354. doi: https://doi.org/10.2478/v10045-008-0044-3.
[11]. Eziashi, E.I., Omamor, I.B. & Odigie, E.E. (2007). Antagonism of Trichoderma viride and effects of extracted water soluble compounds from Trichoderma species and benlate solution on Ceratocystis paradoxa. African Journal of Biotechnology, 6(4): 388-392.
[12]. Viterbo, A., Ramot, O., Chemin, L. & Chet, I. (2002). Significance of lytic enzymes from Trichoderma spp. in the biocontrol of fungal plant pathogens. Antonie Van Leeuwenhoek, 81(1-4): 549-556. doi: 10.1023/A:1020553421740.
[13]. Benítez, T., Rincón, A.M., Limón, M.C. & Codón, A.C. (2004). Biocontrol mechanisms of Trichoderma strains. International Microbiology, 7: 249-260.
[14]. Riker, A.J. & Riker, R.S. (1936). Introduction to research on plant diseases. John Swift and Co., St. Louis, MO. 117.
[15]. Frommer, W., Ager, B., Archer, L., Brunius, G., Collins, C.H., Donikian, R., Frontali, C., et al., (1989).Safety precautions for handling microorganisms of different risk classes. Applied Microbiology and Biotechnology, 30: 541-552.
[16]. Frommer, W., Ager, B., Archer, L., Brunius, G., Collins, C.H., Donikian, R., Frontali, C., Hamp, S., Houwink, E.H., Küenzi, M.T., Krämer, P., Lagast, H., Lund, S., Mahler, J.L., Normand-Plessier, F, Sargeant, K., Tuijenburg Muijs, G., Vranch, S.P. & Werner, R.G. (1989) Safe biotechnology. III. Safety precautions for handling microorganisms of different risk classes. Appl. Microbiol. Biotechnol., 30: 541–552. doi: 10.1007/BF00255357.
[17]. Elad, Y. & Chet, I. (1983). Improved selective media for isolation of Trichoderma spp. Or Fusarium spp. Phytoparasitica, 11: 55–58. doi: 10.1007/BF02980712.
[18]. Sinclair, J.B. & Dhingra, O.D. (1985). Basic plant pathology methods. CRC Press, Boca Raton, FL. 295–315.
[19]. Clarkson, J.P., Payne, T., Mead, A. & Whipps, J.M. (2004). Selection of fungal biological control agents of Sclerotium cepivorum for control of white rot by sclerotial degradation in a UK soil. Plant Pathology, 51: 735-745. doi: 10.1046/j.1365-3059.2002.00787.x
[20]. McLean, K.L., Swaminathan, J., Frampton, C.M., Hunt, J.S., Ridgway, H.J. & Stewart, A. (2005). Effect of formulation on the rhizosphere competence and biocontrol ability of Trichoderma atroviride C52. Plant Pathology, 54: 212-218. doi: 10.1111/j.1365-3059.2005.01158.x.
Published
2017-10-01
How to Cite
Verma, R., Dutta, A., Choudhary, A., & Maurya, S. (2017). Trichoderma asperellum, a potential fungal biocontrol agent against Aspergillus niger. Journal of Advanced Laboratory Research in Biology, 8(4), 74-78. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/285
Section
Articles