Sero-Surveillance of Mycobacterium avium subspecies paratuberculosis Infection in Domestic Livestock in North India Using Indigenous Absorbed Elisa Test
A total of 829 serum samples belonging to domestic livestock (Cattle, buffaloes, goat and sheep) and driven from different parts of North India between 2005 to 2008, were screened to estimate the seroprevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infection using 'indigenous absorbed ELISA kit'. Seroprevalence of MAP in the domestic livestock was 23.1%. Prevalence was higher in large ruminants (24.1%) as compared to small ruminants (22.5%). Highest seropositivity was in cattle (26.9%), followed by goats (23.9%), buffaloes (20.2%), and sheep (19.0%). In cattle region-wise, 25.8, 29.1 and 30.7% animals were positive from Mathura (UP), Rohtak (Haryana), and Bareilly (UP) regions, respectively. In buffaloes, the highest prevalence was found at Bareilly (26.6%) followed by Rohtak (20.0%) and Bhaghpat (18.4%) regions. In goats, 19.6, 37.5, 40.0 and 21.9% animals were positive from Mathura (farm herd), Etawah, Agra and Ajmer (farmers herd) regions, respectively. In sheep, prevalence of MAP was 25.5 and 16.3% in Mathura and Mannavanur regions, respectively. In sheep, prevalence was higher in Northern region as compared to the Southern region of the country. The present study showed that the prevalence of MAP in domestic livestock was moderately higher; therefore there is an urgent need to control the disease at National level in order to improve per animal productivity in the country.
. Chiodini, R.J., Van Kruiningen, H.J., Merkal, R.S., (1984). Ruminant paratuberculosis (Johne’s disease): the current status and future prospects. Cornell. Vet., 74: 217-262.
. Collins, M.T. (2002). Interpretation of a commercial bovine Paratuberculosis Enzyme linked immunosorbent assay by using likelihood ratio. Clin. Diag. Lab. Immunol., 9: 1367.
. Hasonova, L., Pavlik, I. (2006). Economic impact of paratuberculosis in dairy cattle herds: a review. Veterinarni Medicina, 51: 193–211.
. Kumar, P., Singh, S.V., Bhatiya, A.K., Sevilla, I., Singh, A.V., Whittington, R.J., Juste, R.A., Gupta, V.K., Singh, P.K., Sohal, J.S., Vihan, V.S. (2007). Juvenile Capri-Paratuberculosis in India; Incidence and characterization of by six diagnostic tests. Small Rumin. Res., 73: 45-53.
. Rideout, B.A., Brown, S.T., Davis, W.C., Giannella, R.A., Huestan, W.D., Hutchinson, L.J. (2003). Diagnosis and control of Johne’s disease. Washington, DC: National Academies Press; 2003. Available at: http://www.nap.edu/.
. Sharma, G., Singh, S.V., Sevilla, I., Singh, A.V., Whittington, R.J., Juste, R.A., Kumar, S., Gupta, V.K., Singh, P.K., Sohal, J.S., Vihan, V.S. (2008). Evaluation of indigenous milk ELISA with m-culture and m-PCR for the diagnosis of Bovine Johne’s disease (BJD) in lactating Indian dairy cattle. Research in Veterinary Science, 84: 30-37.
. A.V., Singh, S.V., Sohal, J.S., Singh, P.K. (2009). ‘Comparative potential of ‘modified indigenous’, ‘indigenous’ and ‘commercial’ ELISA kits for the diagnosis of Mycobacterium avium subspecies paratuberculosis in goat a sheep. Indian J. of Experimental Biology, 47: 379-382.
. Singh, N., Singh, S.V., Gupta, V.K., Sharma, V.D., Sharma, R.K., Katoch, V.M. (1996). Isolation and identification of Mycobacterium paratuberculosis from naturally infected goat herds in India. Ind. J. Vet. Path., 20: 104-108.
. Singh, S.V., Singh, A.V., Singh, P.K., Sohal, J.S. (2008b). An outbreak of caprine Paratuberculosis in a newly established commercial goat farm. Haryana Vet., 47, pp 110-112.
. Singh, S.V., Singh, A.V., Singh, P.K., Sohal, J.S., Singh, N.P. (2007). Evaluation of an indigenous ELISA for diagnosis of Johne’s disease and its comparison with commercial kits. Indian J. Microbiology, 47: 251-258.
. Singh, S.V., Singh, A.V., Singh, R. Sharma, S., Shukla, N., Misra, S., Singh, P.K., Sohal, J.S., Kumar, H. Patil, P.K., Misra, P., Sandhu, K.S. (2008a). Seroprevalence of Johne’s disease in buffaloes and cattle population of North India using the indigenous ELISA kit based on native Mycobacterium avium subspecies paratuberculosis ‘Bison type’ genotype of goat origin. Comp. Immunol. Microbiol. Infect. Dis., 31: 419-433
. Sivakumar, P., Tripathi, B.N., Singh, N. (2005). Detection of Mycobacterium avium subsp. Paratuberculosis in intestinal and lymph node tissues of water buffaloes (Bubalus bubalis) by PCR and bacterial culture. Veterinary Microbiology, 108: 262-270.
. Vohra, J., Singh, S.V., Singh, A.V., Singh, P.K., Kumar, S., Sohal, J.S. (2008). Comparative distribution of Mycobacterium avium subspecies paratuberculosis in the target and non-target tissues of goats and sheep population in India. Indian J. Ani. Sci., 79: 342-347.
. Whitlock, R.H., Buergelt, C. (1996). Preclinical and clinical manifestations of Paratuberculosis (including pathology). Vet. Clin. North Am. Food Anim. Pract., 12: 345–356.
. Yadav, D., Singh, S.V., Singh, A.V., Sevilla, I., Juste, R.A., Singh, P.K., Sohal, J.S. (2008). Pathogenic ‘Bison-type’ Mycobacterium avium subspecies paratuberculosis genotype characterized from riverine buffalo (Bubalus bubalis) in North India. c 31: 373-87.
. Yokomizo, Y., Merkal, R.S., Lyle, P.A.S. (1983). Enzyme-linked immunosorbent assay for detection of bovine immunoglobulin G1 antibody to a protoplasmic antigen of Mycobacterium paratuberculosis. Am. J. Vet. Res., 44: 2205.
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