Integration of Arbuscular Mycorrhizal Fungi to Grape Vine (Vitis vinifera L.) in Nursery Stage

  • M. Alizadeh Department of Horticulture, Pardis Faculty of Agriculture, Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), P.O. Box 386, Golestan, Gorgan, I.R. Iran.
  • M. Eftekhari Department of Horticulture, Pardis Faculty of Agriculture, Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), P.O. Box 386, Golestan, Gorgan, I.R. Iran.
  • K. Mashayekhi Department of Horticulture, Pardis Faculty of Agriculture, Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), P.O. Box 386, Golestan, Gorgan, I.R. Iran.
  • H. Asghari Shahrood University of Technology, Semnan, Shahroud, I.R., Iran.
  • B. Kamkar Department of Horticulture, Pardis Faculty of Agriculture, Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), P.O. Box 386, Golestan, Gorgan, I.R. Iran.
Keywords: Arbuscular mycorrhizal fungi, Vitis vinifera L., Growth, Biochemical analysis

Abstract

The Arbuscular Mycorrhizal (AM) association is being considered as the commonest Mycorrhizal type involved in grape community. Low population density of these useful fungi in vineyard soil suggests the need for manual inoculation of grapevine plantlets at the nursery stage. The influence of three commercial Arbuscular Mycorrhizal fungi strains (Glomus intraradious, G. mosseae, G. fasciculatus and a mixture of them) on growth and biochemical status of four grapevine varieties (Shahroodi, Asgari, Keshmeshi and Khalili) was investigated under greenhouse conditions. Rooted plantlets derived from hardwood cuttings were transplanted in pots containing leaf mold and sand (1:1) followed by inoculation with different fungal inoculums. Various physiological and biochemical parameters were measured at 30 days intervals. The percentage of root colonization was found to be slightly different amongst inoculated vines but it was found to be significantly different with non-inoculated, control plants. Most growth related parameters (vine length, shoot length and leaf area) were enhanced following Mycorrhization but root length and number of leaves were not significantly affected by any fungal intervention. Treated plants typically showed more obvious modifications in their biochemical status. The chlorophyll content (especially "b" and total), total root and shoot phenols were raised in treated plants. The chlorophyll "a" and total soluble sugars were not statistically different in inoculated and control plants. The overall results of the present study suggest that AM fungi can be manually applied, as an easy and economical approach during nursery production, to boost the physiological and biochemical status of the treated plants and production of high quality healthy plantlets.

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References

[1]. Orcutt, D.M. & Nilsen, E.T. (2000). The physiology of plants under stress. Canada, John Wiley & Sons, INC, p. 110.
[2]. Smith, S.E., Read, & D.J. (1997). Mycorrhizal symbiosis. 2nd edn. Academic Press, San Diego, California, USA. P. 72,106.
[3]. Azcon, R. & Barea, J.M. (1997). Mycorrhizal dependency of a representative plant species in Mediterranean shrublands (Lavandula spica L.) as a key factor to its use for revegetation strategies in desertification-threatened areas. Appl. Soil Ecol., 7: 83-92.
[4]. Lovato, P.E., Schilepp, H., Trouvelot, A. & Gianinazzi, S. (1995). Application of arbuscular mycorrhizal fungi (AMF) in orchard and ornamental plants. In: A. Varma and B. Hock (Editors), Mycorrhiza Structure, Function, Molecular Biology and Biotechnology. Springer, Heidelberg, pp. 521-559.
[5]. Krishna, H., Singh, S.K., Sharma, R.R., Khawale, R.N., Grover, M. & Patel, V.B. (2005). Biochemical changes in micro-propagated grape (Vitis vinifera L.) plantlets due to arbuscular mycorrhizal fungi (AMF) inoculation during ex vitro acclimatization. Sci. Hortic., 106: 554–567.
[6]. Possingham, J.V. & Groot Obbink, J. (1971). Endotrophic mycorrhiza and the nutrition of grape vines. Vitis, 10: 120–130.
[7]. Schreiner, R.P. (2003). Mycorrhizal Colonization of Grapevine Rootstocks under Field Conditions. Am. J. Enol. Vitic., 54(3): 143-149.
[8]. Krishna, H., Singh, S.K., Minakshi, Patel, V.B., Khawale, R.N., Deshmukh, P.S. & Jindal, P.C. (2006). Arbuscular-mycorrhizal fungi alleviate transplantation shock in micro-propagated grapevine (Vitis vinifera L.) Journal of Horticultural Sciences & Biotechnology, 81(2): 259-263.
[9]. Aguín, O., Mansilla, J.P., Vilariño, A. & Sainz, M.J. (2004). Effects of Mycorrhizal Inoculation on Root Morphology and Nursery Production of Three Grapevine Rootstocks. Am. J. Enol. Vitic., 55(1): 108-111.
[10]. Nogales, A., Luque, J., Estaún, V., Camprubí, A., Garcia-Figueres, F. & Calvet, C. (2009). Differential Growth of Mycorrhizal Field-Inoculated Grapevine Rootstocks in Two Replant Soils. Am. J. Enol. Vitic., 60(4): 484-489.
[11]. Phillips, J.M. & Hayman, D.S. (1970). Improved procedure for clearing and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment for infection. Trans. Br. Mycol. Soc., 55: 158–161.
[12]. Nicolson, T.H. (1995). Taxonomy of endomycorrhizal fungi: In Advances in botany; pp. 212-218; (eds. E.G. Mukerji, Binny Mathur. B.P. Chamola and P. Chitralekha (APH Publishing Corporation, New Delhi, India).
[13]. Barnes, J.D., Balaguer, L., Maurigue, E., Elvira, S. & Davison, A.W. (1992). A reappraisal of the use of DMSO for the extraction and determination of chlorophyll ‘a’ and ‘b’ in lichens and higher plants. Environ. Exp. Bot., 32(2): 87–99.
[14]. Malik, C.P. & Singh, M.B. (1980). Plants Enzymology and Histo-Enzymology. Kalyani Publishers, New Delhi, p. 286.
[15]. Hedge, J.E. & Hofreiter, B.T. (1962). In: Carbohydrate Chemistry, 17, (Eds, Whistler, RL. And BeMiller, JN.) Academic Press, New York.
[16]. SAS Institute Inc. (1999-2001). The SAS System for Windows release 8.2. SAS Inst. Inc., Cary, NC, USA.
[17]. Varma, A. & Hock, B. (1998). Mycorrhiza: structure, function, molecular biology, and biotechnology, 2nd Ed, Springer, Germany. P. 452-453.
[18]. Koltai, H., Gadkar, V. & Kapulnik, Y. (2010). Biochemical and practical views of arbuscular mycorrhizal fungus-host association in horticultural crops. Horticultural Reviews, 36: 257-287.
[19]. Bheemareddy, V.S. & Lakshman, H.C. (2009). The effect of the arbuscular mycorrhizae Glomus fasciculatum and Acaulospora laevis on two varieties of Triticum aestivum L. International Journal of plant protection, 2(1): 33-37.
[20]. Toth, R., Page, T. & Castleberry, R. (1984). Differences in mycorrhizal colonization of maize selection for high and low ear leaf phosphorus. Crop Sci., 24: 994-996.
[21]. Graham, J.H., Eissenstat, D.M. & Drouillard D.L. (1991). On the relationship between a plant’s mycorrhizal dependency and rate of vesicular arbuscular mycorrhizal colonization. Funct. Ecol., 5: 773-779.
[22]. Claudia Castillo, R., Leonardo Sotomayor, S., César Ortiz, O., Gina Leonelli, C., Fernando Borie, B. & Rosa Rubio H. (2009). Effect of arbuscular mycorrhizal fungi on an ecological crop of chili peppers (Capsicum annuum L.). Chilean J. Agric. Res., 69(1): 79-87.
[23]. Attia, M. & Eid, R.A. (2005). Effect of inoculation timing with arbuscular mycorrhizal fungi on growth and flowering of micro-propagated chrysanthemum morifolium. Arab Univ. J. Agric. Sci., Ain Shams Univ., Cairo, 13(3): 677-688.
[24]. Estrada-Luna, A.A., Davies Jr. F.T. & Egilla, J.N. (2000). Mycorrhizal fungi enhancement of growth and gas exchange of micro-propagated guava plantlets (Psidium guajava L.) during ex vitro acclimatization and plant establishment. Mycorrhiza, 10: 1-8.
[25]. Van der Heijden, E.W. (2001). Differential benefits of arbuscular mycorrhizal and ectomycorrhizal infection of Salix repens. Mycorrhiza, 10: 185–193.
[26]. Porras Piedra, A., Soriano Martín, M.L., Porras Soriano, A. & Fernández Izquierdo, G. (2005). The influence of arbuscular mycorrhizas on the growth rate of mist-propagated olive plantlets. Span J. Agric. Res., 3(1): 98-105.
[27]. Schiavo, J.A., Martins, M.A. & Rodrigues, e L.A. (2010). Growth of Acacia mangium, Sesbania virgata and Eucalyptus camaldulensis, inoculated with mycorrhizal fungi, under greenhouse conditions and in an area of clay extraction. Acta. Scientiarum Agronomy, 32(1): 171-178.
[28]. Bavaresco, L. & Fogher, C. (1996). Lime-induced chlorosis of grapevine as affected by rootstock and root infection with arbuscular mycorrhiza and Pseudomonas fluorescens. Vitis, 35: 119–123.
[29]. Sheng, M., Tang, M., Chen, H., Yang, B., Zhang, F. & Huang, Y. (2008). Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress. Mycorrhiza, 18: 287–296.
[30]. Giri, B. & Mukerji, K. (2004). Mycorrhizal inoculant alleviates salt stress in Sesbania aegyptiaca and Sesbania grandiflora under field conditions: evidence for reduced sodium and improved magnesium uptake. Mycorrhiza, 14: 307–312.
[31]. Sannazzaro, A.I., Oscar, R., Edgardo, A. & Ana, M. (2006). Alleviation of salt stress in Lotus glaber by Glomus intraradices. Plant Soil, 285: 279–287.
[32]. Colla, G., Rouphael, Y., Cardarelli, M., Tullio, M., Rivera, C.M., & Rea, E. (2007). Alleviation of salt stress by arbuscular mycorrhizal in zucchini plants grown at low and high phosphorus concentration. Biology and Fertility of Soils, 44: 501-509.
[33]. Mathur, N. & A. Vyas, (1995). Influence of VA mycorrhizae on net photosynthesis and transpiration of Ziziphus mauritiana. Journal of plant physiology, 147(3-4): 328-330.
[34]. Mathur, N. & A. Vyas, (1996). Relative efficiency of different VAM fungi on growth and nutrient uptake in Ziziphyus Mauritiana. Indian J. Fores., 19: 129-131.
[35]. Vidhyasekaran, P. (1973). Possible role of ortho-dihydroxy phenolics in grapevine anthracnose disease resistance. Indian J. Exp. Biol., 11(5): 473–475.
[36]. Tang, M., Chen, H. & Shang, H.S. (2000). Mechanism of vesicular–arbuscular mycorrhizal fungi enhanced the resistance of poplar to canker. Scientia. Silvae. Sinica., 36(2): 87–92.
[37]. Ulrichs, C., Fischer, G., Büttner, C. & Mewis, I. (2008). Comparison of lycopene, b-carotene and phenolic contents of tomato using conventional and ecological horticultural practices, and arbuscular mycorrhizal fungi (AMF). Agronomía Colombiana, 26(1): 40-46.
[38]. Devi, M.C. & Reddy, M.N. (2002). Phenolic acid metabolism of groundnut (Arachis hypogaea L.) plants inoculation with VAM fungus and rhizobium. Plant Growth Regulat., 37: 151-156.
[39]. Kapoor, R. (2008). Induced Resistance in Mycorrhizal Tomato is correlated to Concentration of Jasmonic Acid. Online J. Biol. Sci., 8(3): 49-56.
Published
2010-10-01
How to Cite
Alizadeh, M., Eftekhari, M., Mashayekhi, K., Asghari, H., & Kamkar, B. (2010). Integration of Arbuscular Mycorrhizal Fungi to Grape Vine (Vitis vinifera L.) in Nursery Stage. Journal of Advanced Laboratory Research in Biology, 1(2), 79-85. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/37
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