CRP in Relation To CVD

Authors

  • J. Vimalin Hena Department of Microbiology, Hindusthan College of Arts & Science, Coimbatore, India.
  • Ayappan Department of Microbiology, Hindusthan College of Arts & Science, Coimbatore, India.

Keywords:

Atherosclerosis, CRP, Coronary Heart Disease, Cerebrovascular Disease, DAB kit, Peripheral Vascular Disease, Hepatocytes, PBMC, SDS PAGE Gel Electrophoresis

Abstract

Cardiovascular diseases are leading to death in both men and women. One of the risk factors mainly involved in CVD and CRP as inflammatory markers. Atherosclerosis is a disease characterized by chronic inflammation and is now widely accepted to be inflammatory diseases. Clinical manifestations include coronary heart disease [CHD], cerebrovascular disease and peripheral vascular disease. In this study focus on CRP, this protein will be expressed on CVD patients and the relationship between Insulin resistance leads to cardiovascular disease and diabetic diseases occurs in young age & adult age peoples were living worldwide. The CVD patient and normal sample were collected respectively in hospital. These samples were estimated by protein estimation and analysis of CRP using SDS PAGE gel electrophoresis and assay method of western blot done by the color development system using primary & secondary antibody and also DAB kit. The measurements of BMI correlate to the CRP and INSULIN level. Increased BMI that can lead to obesity occurs in both men & women. The CRP protein level increases the chance of cardiovascular disease and insulin level also increased, the patient should develop diabetes. These diseases are analyzed by diagnostic methods. In human CRP was synthesized by hepatocytes and secreted into serum and also whether CRP as a human acute phase protein synthesis by PBMC and isolate the lymphocytes in cultured medium and protein was isolated & estimated due to analysis of CRP using SDS PAGE Gel Electrophoresis. From the preliminary results, there is an evidence of raise in CRP among CVD patients which is to be dealt in detail in the near future to use CRP proteins as a marker in CVD detection.

Downloads

Download data is not yet available.

References

Agrawal, A., Simpson, M.J., Black, S., Carey, M.P. & Samols, D. (2002). A C-reactive protein mutant that does not bind to phosphocholine and pneumococcal C-polysaccharide. J. Immunol., 169(6): 3217–3222. https://doi.org/10.4049/jimmunol.169.6.3217.

Arslanian, S. & Suprasongsin, C. (1996). Insulin sensitivity, lipids, and body composition in childhood: is "syndrome X" present? J. Clin. Endocrinol. Metab., 81(3): 1058–1062. https://doi.org/10.1210/jcem.81.3.8772576.

Aukrust, P., Ueland, T., Lien, E., Bendtzen, K., Müller, F., Andreassen, A.K., Nordøy, I., Aass, H., Espevik, T., Simonsen, S., Frøland, S.S. & Gullestad, L. (1999). Cytokine network in congestive heart failure secondary to ischemic or idiopathic dilated cardiomyopathy. Am. J. Cardiol., 83(3): 376–382. https://doi.org/10.1016/s0002-9149(98)00872-8.

Baumgarten, G., Knuefermann, P. & Mann, D.L. (2000). Cytokines as emerging targets in the treatment of heart failure. Trends Cardiovasc. Med., 10(5): 216–223. https://doi.org/10.1016/s1050-1738(00)00063-3.

Chambers, J.C., Eda, S., Bassett, P., Karim, Y., Thompson, S.G., Gallimore, J.R., Pepys, M.B., & Kooner, J.S. (2001). C-reactive protein, insulin resistance, central obesity, and coronary heart disease risk in Indian Asians from the United Kingdom compared with European whites. Circulation, 104(2): 145–150. https://doi.org/10.1161/01.cir.104.2.145.

Downloads

Published

01-10-2010

How to Cite

Hena, J. V., & Ayappan. (2010). CRP in Relation To CVD. Journal of Advanced Laboratory Research in Biology, 1(2), 104–109. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/42

Issue

Section

Articles