Antagonistic Effects of Lactobacilli on Gram-Negative Bacteria

Authors

  • R. Shanthya Department of Microbiology, Hindusthan College of Arts and Science, Coimbatore-28, India.
  • S. Saranya Department of Microbiology, Hindusthan College of Arts and Science, Coimbatore-28, India.
  • N. Hema Shenpagam Department of Microbiology, Hindusthan College of Arts and Science, Coimbatore-28, India.

Keywords:

Antagonism, Gram-negative bacteria, Lactic acid bacteria, Well diffusion method

Abstract

Three lactobacilli strains were examined for the inhibitory activity against some gram-negative bacteria with a well diffusion method. Lactobacilli have strongest antagonistic activity against P. aeruginosa in well diffusion method; it was the least sensitive tested bacteria. In spite of E. coli is inhibited secondly in well diffusion method (16mm), the value of the inhibition is lower than spot on lawn method (26mm). These results showed that spot on lawn method is a better method than the well diffusion method.

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References

Schuenzel, K.M. & Harrison, M.A. (2002). Microbial antagonists of foodborne pathogens on fresh, minimally processed vegetables. J. Food Prot., 65(12): 1909–1915. https://doi.org/10.4315/0362-028x-65.12.1909.

Mossel, D.A.A., Corry, J.E.L., Struijk, C.B. & Baird, R.M. (1995). Essentials of the Microbiology of Foods: a Textbook for Advanced Studies. John Wiley and Sons, Chichester, pp. 735.

Holt, G.J., Krieg, N.R., Sneath, P.H.A., Staley, J.T. & Williams, S.T. (1994). Bergey's manual of determinative bacteriology. 9th ed., Williams & Wilkins, Baltimore, p. 566.

Daeschel, M.A. (1989). Antimicrobial substances from lactic acid bacteria for use as food preservatives. Food Technol., 43(1): 164-167.

Klaenhammer, T.R. (1988). Bacteriocins of lactic acid bacteria. Biochimie, 70(3): 337–349. https://doi.org/10.1016/0300-9084(88)90206-4.

Rodriguez, J.M., Sobrino, O.J., Fernandez, M.F., Hernandez, P.E. & Sanz, B. (1989). Antimicrobial activity of lactic acid bacteria isolated from Spanish dry fermented sausages. In: Proceedings of the 35th International Congress of Meat Science and Technology, Copenhagen, pp. 308-312.

Ross, K.F., Ronson, C.W. & Tagg, J.R. (1993). Isolation and characterization of the lantibiotic salivaricin A and its structural gene salA from Streptococcus salivarius 20P3. Appl. Environ. Microbiol., 59(7): 2014–2021. https://doi.org/10.1128/aem.59.7.2014-2021.1993.

Cadirci, B.H. & Çitak, S. (2005). A Comparison of Two Methods Used for Measuring Antagonistic Activity of Lactic Acid Bacteria. Pak. J. Nutr., 4(4): 237-241. https://dx.doi.org/10.3923/pjn.2005.237.241.

Kivanç, M. (1990). Antagonistic action of lactic cultures toward spoilage and pathogenic microorganisms in food. Die Nahrung, 34(3): 273–277. https://doi.org/10.1002/food.19900340318.

Schillinger, U. & Lücke, F.K. (1989). Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol., 55(8): 1901–1906. https://doi.org/10.1128/aem.55.8.1901-1906.1989.

Con, A.H. & Gökalp, H.Y. (2000). Production of bacteriocin-like metabolites by lactic acid cultures isolated from sucuk samples. Meat Sci., 55(1): 89–96. https://doi.org/10.1016/s0309-1740(99)00129-1.

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Published

01-04-2011

How to Cite

Shanthya, R., Saranya, S., & Shenpagam, N. H. (2011). Antagonistic Effects of Lactobacilli on Gram-Negative Bacteria. Journal of Advanced Laboratory Research in Biology, 2(2), 70–72. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/66

Issue

Vol. 2 No. 2 (2011): April

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Articles

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