Antagonistic Effects of Lactobacilli on Gram-Negative Bacteria
Keywords:
Antagonism, Gram-negative bacteria, Lactic acid bacteria, Well diffusion methodAbstract
Three lactobacilli strains were examined for the inhibitory activity against some gram-negative bacteria with a well diffusion method. Lactobacilli have strongest antagonistic activity against P. aeruginosa in well diffusion method; it was the least sensitive tested bacteria. In spite of E. coli is inhibited secondly in well diffusion method (16mm), the value of the inhibition is lower than spot on lawn method (26mm). These results showed that spot on lawn method is a better method than the well diffusion method.
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References
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[3]. Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T. (1994). Bergey’s Manual of Determinative Bacteriology, 9th edition. Williams and Wilkins, 566,
[4]. Daeschel, M.A. (1989). Antibacterial substances from lactic acid bacteria for use as food preservatives. Food Technol., 43, 164-167,
[5]. Klaenhammer, T.R. (1988). Bacteriocins of lactic acid bacteria. Biochimie, 70, 337-349.
[6]. Rodriguez, J.M., Sobrino, O.J., Fernandez, M.F., Hernandez, P.E., Sanz, B. (1989). Antimicrobial activity of lactic acid bacteria isolated from Spanish dry fermented sausages, 35th Int. Congr. Meat Sci. Technol. Copenhagen: 308-312.
[7]. Ross, K.F., Ronson, C.W., Tagg, J.R. (1993). Isolation and characterization of the lantibiotic salivaricin A and its structural gene salA from Streptococcus salivarius 20P3. Appl. Environ. Microb., 59(7): 2014-2021.
[8]. Cadirci, B.H., Citak, S. (2005). A comparison of two methods used for measuring antagonistic activity of lactic acid bacteria. Pak. J. Nutr., 4 (4):237-241.
[9]. Kivanç, M. (1990). The antagonistic action of lactic cultures toward spoilage and pathogenic microorganisms in food. Die Nahrung., 34(3): 273-277.
[10]. Schillinger, U., Lucke, F.K. (1989). Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol., 55(8):1901-1906.
[11]. Con, A.H., Gokalp, H.Y. (2000). Production of bacteriocin-like metabolites by lactic acid cultures isolated from sucuk samples. Meat Sci., 55: 89-96.
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Published
01-04-2011
How to Cite
Shanthya, R., Saranya, S., & Shenpagam, N. H. (2011). Antagonistic Effects of Lactobacilli on Gram-Negative Bacteria. Journal of Advanced Laboratory Research in Biology, 2(2), 70–72. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/66
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