Optimization of Growth Regulators for Induction of Callus from Cotton (Gossypium hirsutum)
Keywords:Gossypium hirsutum L., Epicotyl, cotyledon, MS (Murashige and Skoog) media, 1-naphthaleneacetic acid (NAA), 6-Benzylaminopurine (BAP), Kinetin (KIN) and 2,4-dichlorophenoxyacetic acid (2,4–D), Callus
The objective of this study was to optimize the concentration of different plant growth regulators or hormones for callus induction of cotton (Gossypium hirsutum L.). Different types and concentrations of growth regulators were tested in order to obtain the best callus formation. Four growth hormones such as 1-naphthaleneacetic acid NAP), 6-Benzylaminopurine (BAP), Kinetin and 2,4-dichlorophenoxyacetic acid (2,4–D) were used in this study. It was found that growth regulator type and concentration had a significant effect on the callus induction, the increment of callus index and callus physical appearance. The higher frequency of callus growth (95-100%) were observed on both epicotyls and cotyledon explants cultured on basal medium supplemented with 0.1mg/l NAA + 0.5mg/l Bap and various concentrations such as 0.2+0.1, 0.5+0.1, 1.0+0.2mg/l of NAA + BAP also shows good callus response but at higher concentration of the same hormones shuts the callus growth. The concentration of BAP and 2,4-D also shows good callus response in higher concentration whereas low concentrations of this hormone combination show nil effect. The morphology of callus differs upon the hormonal concentration from green to white and green to brown with various textures. This protocol paves the way for the development of in vitro regeneration for cotton and consequently will promote the application of plant tissue culture.
Abdellatef, E. & Khalafallah, M.M. (2007). Adventitious shoot formation and plant regeneration in medium staple cotton (Gossypium hirsutum L. cv Barac (67) B). Int. J. Agri. Biol., 9(6): 913- 916.
Rashid, B., Husnain, T. & Riazuddin, S. (2004). In vitro Shoot Tip Culture of Cotton (Gossypium hirsutum). Pak. J. Bot., 36(4): 817-823.
Davidonis, G.H. & Hamilton, R.H. (1983). Plant regeneration from Callus tissue of Gossypium hirsutum L. Plant Sci. Lett., 32: 89-93.
Finer, J.J. (1988). Plant regeneration from somatic embryogenic suspension cultures of cotton (Gossypium hirsutum). Plant Cell Rep., 7: 399-402.
Flint, H.M., Henneberry T.J., Wilson, F.D., Holguin, E., Paries, N. & Buchler, R.E. (1995). The effects of transgenic cotton Gossypium hirsutum L., containing Bacillus thuringiensis toxin genes for the control of pink bollworm, Pectinophora gossypiella (Saunders) and other arthropods. South Western Entomologists, 20: 281-292.
Hemphill, J.K., Maier, C.G.A. & Chapman, K.D. (1998). Rapid in-vitro plant regeneration of cotton (Gossypium hirsutum L.). Plant Cell Rep., 17(4): 273–278. https://doi.org/10.1007/s002990050391.
Sakhanokho, H.F., Zipf, A., Rajasekaran, K., Saha, S. & Sharma, G.C. (2001). Induction of Highly Embryogenic Calli and Plant Regeneration in Upland (Gossypium hirsutum L.) and Pima (Gossypium barbadense L.) Cottons. Crop Sci., 41(4): 1235–1240. https://doi.org/10.2135/cropsci2001.4141235x.
Trolinder, N.L. & Goodin, J.R. (1987). Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep., 6: 231-234.
Wilson, D.F., Flint, H.M., Deaton, R.W., Fischoff, D.A., Perlak, F.J., Armstrong, T.A., Fuchs, R.L., Berberich, S.A., Parks, N.J. & Stapp, B.A. (1992). Resistance of cotton lines containing a Bacillus thuringiensis toxin to Pink Bollworm (Lepidoptera: Gelechiidae) and other insects. J. Econ. Entomol., 85: 1516-1521.
Zhang, B.-H., Feng, R., Liu, F. & Wang, Q. (2001). High frequency somatic embryogenesis and plant regeneration of an elite Chinese cotton variety. Bot. Bull. Acad. Sin., 42: 9-16.
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