Syzygium cumini Leaf Extract Showed Vibriocidal Activity on Selected Diarrhea Causing Bacteria

Authors

  • Nilanjana Paul Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh.
  • Anwarul A. Akhand Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh.
  • Sahab Uddin Babu Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh.
  • Nazrul Islam Laboratory Science Division, ICDDR, B, Mohakhali, Dhaka-1212, Bangladesh.
  • Nazmul Ahsan Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh.

Keywords:

Plant extract, Vibrio, Cholera, Vibriocidal activity

Abstract

The objective of the study was to investigate the effect of ethanolic leaf extract (ELE) of Syzygium cumini against Vibrio cholerae particularly two serogroups Ogawa and Inaba. The phenolic content of the ELE was found high which is comparable to ascorbic acid. Brine shrimp lethality bioassay was then performed to check the cytotoxic effects of ELE. The lower LC50 value of ELE obtained indicated its less cytotoxic properties. The antimicrobial activity of the extract was then evaluated by the disc diffusion method against multi-drug resistant Vibrio serogroups Ogawa and Inaba. The extract effectively inhibited the growth of both serogroups. Altogether, the results demonstrated that the ELE of S. cumini has a significant vibriocidal activity that might be useful as a drug for the treatment of cholera.

Downloads

Download data is not yet available.

References

WHO (2010). Cholera factsheet 107. World Health Organization, Geneva. Available at: http://www.who.int/mediacentre/factsheets/fs107/en/index.html.

CDC (2010). Outbreak notice: cholera in Haiti. Centers for Disease Control and Prevention, Atlanta, GA.

Albert, M.J., Ansaruzzaman, M., Bardhan, P.K., Faruque, A.S.G., Faruque, S.M., Islam, M.S., Mahalanabis, D., Sack, R.B., Salam, M.A., Siddique, A.K., Yunus, M.D. & Zaman, K. (1993). Large epidemic of cholera-like disease in Bangladesh caused by Vibrio cholerae 0139 synonym Bengal. Lancet, 342: 387–390. https://doi.org/10.1016/0140-6736(93)92811-7.

Ramachandran, D., Bhanumathi, R. & Singh, D.V. (2007). Multiplex PCR for detection of antibiotic resistance genes and the SXT element: application in the characterization of Vibrio cholerae. J. Med. Microbiol., 56(3): 346–351. https://doi.org/10.1099/jmm.0.46655-0.

Moresco, R.N., Sperotto, R.L., Bernardi, A.S., Cardoso, R.F. & Gomes, P. (2007). Effect of the aqueous extract of Syzygium cumini on carbon tetrachloride-induced hepatotoxicity in rats. Phytother. Res., 21(8): 793–795. https://doi.org/10.1002/ptr.2158.

Gowri, S.S. & Vasantha, K. (2010). Phytochemical screening and antibacterial activity of Syzygium cumini (L.) (Myrtaceae) leaves extracts. Int. J. PharmTech Res., 2(2): 1569-1573.

Singleton, V.L. & Rossi, J.A. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. Am. J. Enol. Vitic., 16(3): 144–158.

Meyer, B.N., Ferrigni, N.R., Putnam, J.E., Jacobsen, L.B., Nichols, D.E. & McLaughlin, J.L. (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med., 45(5): 31–34. https://doi.org/10.1055/s-2007-971236.

Perez, C., Paul, M. & Bazerque, P. (1990). An antibiotic assay by the agar-well diffusion method. Acta Biol. Med. Exp., 15: 113-115.

Bauer, A.W., Kirby, W.M., Sherris, J.C. & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 45(4): 493–496.

Hatano, T., Edamatsu, R., Hiramatsu, M., Mori, A., Fujita, Y., Yasuhara, T., Yoshida, T. & Okuda, T. (1989). Effects of the Interaction of Tannins with Co-existing Substances. VI. : Effects of Tannins and Related Polyphenols on Superoxide Anion Radical, and on 1, 1-Diphenyl-2-picrylhydrazyl Radical. Chem. Pharm. Bull., 37(8): 2016–2021. https://doi.org/10.1248/cpb.37.2016.

Duh, P.D., Tu, Y.Y. & Yen, G.C. (1999). Antioxidant Activity of Water Extract of Harng Jyur (Chrysanthemum morifolium Ramat). LWT - Food Sci. Technol., 32(5): 269–277. https://doi.org/10.1006/fstl.1999.0548.

Benherlal, P.S. & Arumughan, C. (2007). Chemical composition and in vitro antioxidant studies on Syzygium cumini fruit. J. Sci. Food Agric., 87(14): 2560–2569. https://doi.org/10.1002/jsfa.2957.

Ruan, Z.P., Zhang, L.L. & Lin, Y.M. (2008). Evaluation of the antioxidant activity of Syzygium cumini leaves. Molecules, 13(10): 2545–2556. https://doi.org/10.3390/molecules13102545.

Anibijuwon, I.I. & Udeze, A.O. (2009). Antimicrobial Activity of Carica papaya (Pawpaw Leaf) on Some Pathogenic Organisms of Clinical Origin from South-Western Nigeria. Ethnobotanical Leaflets, 13: 850-864.

Downloads

Published

03-07-2011

How to Cite

Paul, N., Akhand, A. A., Babu, S. U., Islam, N., & Ahsan, N. (2011). Syzygium cumini Leaf Extract Showed Vibriocidal Activity on Selected Diarrhea Causing Bacteria. Journal of Advanced Laboratory Research in Biology, 2(3), 108–111. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/71

Issue

Section

Articles