Involvement of HLA in Serum Beta-2 Microglobulin levels among Mumbai HIV-1 Patients

Authors

  • U. Shankarkumar National Institute of Immunohaematology, 13th Floor, KEM Hospital, Parel, Mumbai - 400012, Maharashtra, India.
  • A. Shankarkumar National Institute of Immunohaematology, 13th Floor, KEM Hospital, Parel, Mumbai - 400012, Maharashtra, India.
  • K. Ghosh National Institute of Immunohaematology, 13th Floor, KEM Hospital, Parel, Mumbai - 400012, Maharashtra, India.

Keywords:

HIV patients, B2M, HLA, Mumbai

Abstract

HLA allele involves along with B-2 microglobulin (B2M) and T cell receptor in host specific immune response. HLA alleles are known for its extensive polymorphism in order to accommodate the maximum of antigenic peptides. Serum beta-2 microglobulin has been reported to have a prognostic value for predicting disease progression in HIV patients. In this study, we evaluated the serum B2M levels and the HLA profile among the 124 HIV patients and 94 Normal healthy controls from Mumbai. The B2M levels were estimated by conventional ELISA technique while the HLA A and HLA B were done using PCR – SSOP molecular kits. Our results revealed that HIV patients showed increased B2M levels (88.70%) range (2.5ug/ml – 60ug/ml) compared the controls (42.55%). HLA A*11:01:01 (OR=3.25; P value 0.0015), HLA B*35:01:01 OR=3.57;P value 0.0002), HLA B*44:03:01 (OR=5.32; P value 0.0036), haplotypes HLA A*11:01:01- B*35:01:01 (OR=5.68; P value 2.47E-05), HLA A*33:03:01- B* 44:03:01 (OR=19.95; P value 0.0003) were significantly increased among the B2M increased HIV patients. While HLA A*03:01:01:01(OR=0.18; P value 0.0061) was significantly decreased among B2M increased HIV patients. Our results reveal that the production of serum B2M levels correlates with the HIV disease progression. Therefore it could be of a prognostic value in HIV infection.

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References

[1]. Sonkar, G.K., Usha, Singh R.G. (2008). A preliminary study on the significant value of beta-2-microglobulin over serum creatine in renal transplant rejection and renal failure. Singapore Med. J., 49:786-9.
[2]. Miller, S.A., Dykes, D.D., Polesky, H.F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res., 16: 1215
[3]. Mack, S.J., Hollenbach, J.A. (2010). Allele Name Translation Tool and update Nomenclature software tools for automated translation of HLA names between successive nomenclatures. Tissue Antigens, 75: 457-461.
[4]. Cooper, E.H., Forbes, M.A., Hammbling, M.H. (1984). Serum beta 2-microglobulin and C reactive protein considerations in viral infections. J. Clin. Pathol., 37:1140-1143.
[5]. Backman, L., Ringden, O., Bjorkhem, I., Lindback, B. (1986). Increased serum beta 2 microglobulin during rejection, cyclosporine-induced nephrotoxicity and cytomegalovirus infection in renal transplant recipients. Transplantation, 42:368-371.
[6]. Corlin, D.B., Sen, J.W., Ladefoged, S., Lund, G.B., Nissen, M.H., Heegaard, N.H. (2005). Quantification of cleaved beta 2 –microglobulin in serum from patients undergoing chronic hemodialysis. Clin. Chem., 51: 1177-1184.
[7]. Jovanovic, D., Krstivojevic, P., Obradovic, I., Durdevic, V., Dukanovic, L. (2003). Serum cystatin C and beta 2-microglobulin as markers of glomerular filtration rate. Renal Fail., 25: 123-133.
[8]. Child, J.A., Kushwaha, M.R. (1984). Serum beta 2-microglobulin in lymphoproliferative and myeloproliferative diseases. Hematol. Oncol., 2: 391-401.
[9]. Garden, G.A., Moss, G.B., Emonyi, W., Bwayo, J., Velentgas, P., Kreiss, J. (1993). Beta 2 microglobulin as a marker of HIV disease status in Nairobi, Kenya. Int. J. STD AIDS, 4: 49-51.
[10]. Shankarkumar, U., Thakar, M., Mehendale, S., Praranjape, R.S., Mohanty, D. (2003). Association of HLA B*3520, B*1801 and Cw*1507 with HIV-1 infection Maharashtra, India. J. Acquir. Immune Defic. Syndr., 34: 113-114.
[11]. Shankarkumar, U. (2004). HIV and HLA association Indian scenario. Journal of HIV Therapy, 9: 60-64.
[12]. Shankarkumar, U., Pawar, A., Ghosh, K. (2007). Specific Human Leukocyte antigen alleles associated with HIV-1 infection in Indian population. J. Acquir. Immune Defic. Syndr., 44: 489-490.

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Published

03-07-2011

How to Cite

Shankarkumar, U., Shankarkumar, A., & Ghosh, K. (2011). Involvement of HLA in Serum Beta-2 Microglobulin levels among Mumbai HIV-1 Patients. Journal of Advanced Laboratory Research in Biology, 2(3), 126–128. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/75

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