Effect of Adhatoda vasica (L.) Nees Leaf Extract Prepared by two Different Methods on Mitosis of Root Meristematic Cells of Allium cepa L.
Keywords:Adhatoda vasica, Leaf Extract, Allium cepa, Genotoxicity, Cytotoxicity
Adhatoda vasica is a well-known plant drug in Ayurvedic and Unani medicine. Adhatoda leaves have been used extensively in Ayurvedic Medicine primarily for respiratory disorders. Aqueous leaf extract of A. vasica was used to investigate its cytotoxic and genotoxic effects on root meristematic cells of Allium cepa. 1, 2.5, 5, 10 and 20% concentrations of leaf extract prepared by two different ways were used in the present study. It was found that leaf extract of A. vasica exhibits mitodepressive activity as well as it induces disorder of chromosome kinetic including formation of sticky chromosome, c-metaphase, metaphasic and anaphasic disorders in Allium cepa root meristem.
. Amin, A.W. (2002). Cytotoxicity Testing of Sewage Water Treatment Using Allium cepa Chromosome Aberrations Assay. Pak. Jour. Biologic Sci., 5 (2):184-188.
. Atal, C.K. (1980). Chemistry and Pharmacology of vasicine- A new oxytocic and abortifacient. Regional Research laboratory, Jammu-Tawi.
. Bakare, A., and Osibanjo, O. (2000). Effect of simulated leachate on chromosomes and mitosis in roots of Allium cepa (L). J. Environ. Biol., 21(3):263–271
. Bose, C.K. (1932). Pharmacopoeia indica. In: Being a Collection of Vegetable Mineral and Animal Drugs in Common Use in India. The Book Company, Calcutta, pp. 24–25.
. Chakraborty, A. and Brantner, A.H. (2001). Study of alkaloids from Adhatoda vasica Nees on their anti-inflammatory activity. Phytother. Res., 15 (6): 532-534.
. Chopra, R.N. (1955). A Review of Work on Indian Medicinal Plants. Indian Council of Medical Research, New Delhi, p.23.
. Clamp, J.R., M. Hartog, M. and J.H. Shelley (1979). Carbohydrate-containing materials in urine from normal and diabetic subjects. Clin. Sci. (Lond.), 56: 193-196.
. Darrel Crain, D.C. (2007). Mysterious Disappearance of Bees. Planet Chiropractic News, Monday, July 09. 2007.
. Gao, H., Huang, Y.N., Gao, B. Li, P., Inagaki, C. and Kawabata, J. (2008). Inhibitory effect on ?-glucosidase by Adhatoda vasica Nees. Food Chem., 108: 965–972.
. Grant, W.F. (1982). Chromosome aberrations assay in Allium. A report of the U.S. Environmental protection Agency Gene-Tox programme. Mut. Res., 99:273-291.
. Ilango, K., Chitra, V., Kanimozhi, P. and Balaji, G. (2009). Antidiabetic, antioxidant and antibacterial activities of leaf extracts of Adhatoda zeylanica Medic (Acanthaceae). J. Pharm. Sci. & Res., 1(2): 67-73.
. Indian Herbal Pharmacopoeia (Revised new edition) Mumbai, India: Indian Drug Manufacturing Association; (2002). pp. 33–39.
. Malla, B.S., Rajbhandari, B.S., Shrestha, B.T., Adhikari, M.P., Adhikari, R.S. (1982). Wild edible plants of Nepal. Department of Medicinal Plants, Nepal, Bulletin No. 9. Kathmandu, pp. 3-4.
. Maluszynska, J. and Juchimiuk, J. (2005). Plant Genotoxicity: A molecular cytogenetic approach in plant bioassays. Arhiv za Higijenu Rada i Toksikologiju, 56, 177-184.
. Manjunath, B.L. (1948). The Wealth of India, A Dictionary of Indian Raw Materials and Industrial Products. Council of Scientific and Industrial Research, Delhi, pp. 31-32
. Mercykutty, V.C. and Stephen, J. (1980). Adriamycin induced genetic toxicity as demonstrated by Allium test. Cytologia, 45: 769-777.
. Nath, D., Sethi, N., Srivastava, S., Jain, A.K., Srivastava, R. (1997). Survey on indigenous medicinal plants used for abortion in some districts of Uttar Pradesh. Fitoterapia, 68: 223-225.
. Padmaja, M., Sravanthi, M. and Hemalatha, K.P.J. (2001). Evaluation of antioxidant activity of two Indian medicinal plants. Journal of Phytology, 3(3): 86-91.
. Schneiderman, M.H., Dewey, W.C. and Highfield, D.P. (1971). Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 with cycloheximide. Exp. Cell Res., 67; 147- 155.
. Schulze, E. and Kirscher, S. (1996). Microtubule dynamics in interphase Cells. J. Cell Biol., 102:1020–1031.
. Sharma, A.K. and Sharma, A. Chromosome Techniques- Theory and Practice. Butterworth, (1983).
. Soliman, M.I. (2001). Genotoxicity testing of neem plant (Azadirachta indica A. Juss) using the Allium cepa chromosome aberration assays. Online J. Biol. Sci., 1(11):1021-1027.
. Soni, S., Anandjiwala, S., Patel, G., Rajani, M. (2008). Validation of different methods of preparation of Adhatoda vasica leaf juice by quantification of total alkaloids and vasicine. Indian J. Pharm. Sci., 70: 36-42.
. Srinivasarao, D., Jayarraj, I.A., Jayraaj, R. and Prabha, M.L. (2006). A study on antioxidant and anti-inflammatory activity of vasicine against lung damage in rats. Indian J. Allergy Asthma Immunol., 20(1): 1-7.
. Turkoglu, S. (2007). Genotoxicity of five food preservatives tested on root tips of Allium cepa L. Mutat. Res., 626: 4-14.