Effect of Fusarium sp. On Paddy Straw Digestibility and Biogas Production


  • Urmila Gupta Phutela School of Energy Studies for Agriculture, College of Agricultural Engineering and Technology, Punjab Agricultural University, Ludhiana, Punjab, India.
  • Nidhi Sahni Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana-141004, Punjab, India.


Biodegradation, Biogas production, Lignin, Paddy straw, Fusarium sp., Silica


Chopped and moist paddy straw was pretreated with Fusarium sp. to enhance its digestibility and biogas production. The potential of microbial pretreatment of paddy straw was investigated at regular interval of 5, 10, 15 and 20 days by determining the change in Chemical composition of paddy straw like cellulose, hemicellulose, lignin and silica contents. The pretreated straw was used for biogas production in 2l capacity biogas digesters. Results indicate that the cellulose, hemicellulose, lignin and silica contents decreased by 17.2%, 3.4%, 27.1% & 16.5% respectively. Biogas production also increased by 53.8% in 10 days pretreated samples. The significantly higher reduction of silica along with lignin content in the pretreated straw indicates that removal of silica by Fusarium sp. might be more responsible for increasing paddy straw digestibility and biogas production.


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[1]. Anonymous, (2010). Ministry of Agriculture, Ministry of External Affairs, Government of India. (http://www.indiabusiness.nic.in/economy/agriculture.html) 1/5/2010.
[2]. AOAC, (2000). Official methods of analysis of AOAC International, 17th Edition, Maryland, USA. Association of Analytical Communities.
[3]. Bisaria, V.S. and Ghose, T.K. (1981). Biodegradation of cellulosic materials: Substrates, microorganisms, enzymes and products. Enzyme Microb. Technol., 3:90-104.
[4]. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956). Calorimetric method for determination of sugars and related substances. Anal. Chem., 28: 350-56.
[5]. Ehrlich, H.L. (2006). Geomicrobiology: Relative role of bacteria and fungi as geomicrobial agent. In: Gadd GM (ed) Fungi in biogeochemical cycle. Cambridge University Press, Cambridge, UK. pp 1-27.
[6]. Jafari, M.A., Nikkhah A., Sadeghi, A.A. and Chamani, M. (2007). The effect of Pleurotus spp. on chemical composition and in vitro digestibility of rice straw. Pak. J. Biol. Sci., 10 (15): 2460-64.
[7]. Jalc, D., Nerud, F. and Siroka, P. (1998). The effectiveness of biological treatment of wheat straw by white-rot fungi. Folia Microbiol., 43(6): 687-89.
[8]. Jenkins, B.M. and Bhatnagar, A.P. (2003). On the electric power potential from paddy straw in the Punjab and the optimal size of the power generation station. Bioresour. Technol., 37: 35-41.
[9]. Kashyap, D.R., Dadhich, K.S. and Sharma. S.K. (2003). Biomethanation under psychrophilic conditions: a review. Bioresour. Technol., 87:147-53.
[10]. Keller, F.A., Hamilton, J.E. and Nguyen, Q.A. (2003). Microbial pretreatment of biomass: potential for reducing severity of thermochemical biomass pretreatment. Appl. Biochem. Biotechnol., 105: 27-41.
[11]. Kirk, T.K. (1984). In: Nicholas, D.D. (ed.) Wood Determination and its prevention by preservative treatments, University Press, New York Syracuse. 149-81.
[12]. Maiorella, B.L. (1985). Ethanol fermentation, In: Young, M. (ed.) Comprehensive biotechnol. Pergamon Press, Oxford. 3:861-914.
[13]. Mandhulika, Singh, D.P. and Malik, R.K. (1993). Isolation of a few lignocelluloses degrading fungi. Ind. J. Microbiol., 33:265-67.
[14]. Mehta, V., Gupta, J.K. and Kaushal, S.C. (1990). Cultivation of Pleurotus florida mushroom on rice straw and biogas production from spent straw. World J. Microbiol. Biotechnol., 6 (4): 366-70.
[15]. Okano, K., Kitagawa, M., Sasaki, Y. and Watanabe, T. (2005). Conversion of Japanese red cedar (Cryptomeria japonica) into a feed for ruminants by white-rot basidiomycetes. Anim. Feed Sci. Technol., 120:235-43.
[16]. Pauss, A., Naveau, H. and Nyns, E.J. (1987). Biogas Production, In: Hall, D.O. and Overend, R.P. (eds.) Biomass, A Wiley-Inter-science Publication, Great Britain, 273-91.
[17]. Saratale, G.D., Chen, S.D., Lo, Y.C., Saratale, R.G. and Chang, J.S. (2008). Outlook of biohydrogen production from lingocellulosic feedstock using dark fermentation-a review. J. Sci. Ind. Res., 67:962-79.
[18]. Schurz, J. and Ghose, T.K. (1978). Bioconversion of cellulosic substances into energy chemicals and Microbial. Protein Proc. Symp. (IIT, New Delhi) 37.
[19]. Shi, J., Shivappa, R.R.S., Chinn, M. and Howell, N. (2009). Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production. Biomass Bioener., 33:88-96.
[20]. Sinegani, A.A.S., Emtiazi, G., Hajrasuliha, S. and Shariatmadar, H. (2005). Biodegradation of some agricultural residues by fungi in agitated submerged cultures. African J. Biotechnol., 4: 1058-61.
[21]. Taniguchi, M., Suzuki, H., Watanabe, D., Sakai, K., Hoshino, K., Tanaka, T. (2005). Evaluation of pretreatment with Pleurotus ostreatus for enzymatic hydrolysis of rice straw. J. Bioscience Bioeng., 100:637-43.
[22]. Wang, J. and Christopher, S.A. (2003). Intercomparison between satellite-derived aerosol optical thickness and PM2.5 mass: Implications for air quality studies. Geophysics Research Letters, 30:2095-98.
[23]. Zafar, S.I., Kausar, T.K. and Shah. F.H. (1980). Biodegradation of cellulose component of rice straw by Pleurotus sajor-caju. Folia Microbiol., 26:394-97.
[24]. Zhang, R., Li, X. and Fadel, J.G. (2002). Oyster mushroom cultivation with rice and wheat straw. Bioresour. Technol., 82:277-84.




How to Cite

Phutela, U. G., & Sahni, N. (2012). Effect of Fusarium sp. On Paddy Straw Digestibility and Biogas Production. Journal of Advanced Laboratory Research in Biology, 3(1), 9–12. Retrieved from https://e-journal.sospublication.co.in/index.php/jalrb/article/view/87